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枯草芽孢杆菌芽孢形成过程中转录复合物的演变

Evolution of the transcription complex during sporulation of Bacillus subtilis.

作者信息

Brevet J

出版信息

J Bacteriol. 1976 Jan;125(1):74-83. doi: 10.1128/jb.125.1.74-83.1976.

Abstract

Ribonucleic acid polymerase activity in partially purified extract of cells of Bacillus subtilis harvested at different times (t-1, to, t1, and t2) was studied by zone centrifugation. During the course of sporulation, vegetative sigma-factor activity decreased and the transcription complex lost some of its affinity for active sigma factor. The complex underwent a two-stage change in sedimentation value, from 14.5S in vegetative growth phase to a 13S species very early in sporulation to a 16S species at later times. Two SpoO mutants have been studied by zone centrifugation. One strain, a rifampin-resistant (RfmR) mutant, failed to show any modification of the transcription complex, whereas the other, a Rfms strain, underwent a partial evolution of the transcription complex after to.

摘要

通过区带离心法研究了在不同时间(t-1、t0、t1和t2)收获的枯草芽孢杆菌细胞部分纯化提取物中的核糖核酸聚合酶活性。在芽孢形成过程中,营养型σ因子活性下降,转录复合物对活性σ因子的亲和力丧失。该复合物在沉降值上经历了两个阶段的变化,从营养生长阶段的14.5S到芽孢形成早期的13S种类,再到后期的16S种类。通过区带离心法研究了两个SpoO突变体。一个菌株是耐利福平(RfmR)突变体,未显示转录复合物有任何变化,而另一个Rfms菌株在t0之后转录复合物发生了部分演变。

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本文引用的文献

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Subunit composition of B. subtilis RNA polymerase.
Nature. 1970 Jun 27;226(5252):1244-5. doi: 10.1038/2261244a0.
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Catabolic repression of bacterial sporulation.细菌芽孢形成的分解代谢阻遏
Proc Natl Acad Sci U S A. 1965 Sep;54(3):704-11. doi: 10.1073/pnas.54.3.704.

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