Brevet J
J Bacteriol. 1976 Jan;125(1):74-83. doi: 10.1128/jb.125.1.74-83.1976.
Ribonucleic acid polymerase activity in partially purified extract of cells of Bacillus subtilis harvested at different times (t-1, to, t1, and t2) was studied by zone centrifugation. During the course of sporulation, vegetative sigma-factor activity decreased and the transcription complex lost some of its affinity for active sigma factor. The complex underwent a two-stage change in sedimentation value, from 14.5S in vegetative growth phase to a 13S species very early in sporulation to a 16S species at later times. Two SpoO mutants have been studied by zone centrifugation. One strain, a rifampin-resistant (RfmR) mutant, failed to show any modification of the transcription complex, whereas the other, a Rfms strain, underwent a partial evolution of the transcription complex after to.
通过区带离心法研究了在不同时间(t-1、t0、t1和t2)收获的枯草芽孢杆菌细胞部分纯化提取物中的核糖核酸聚合酶活性。在芽孢形成过程中,营养型σ因子活性下降,转录复合物对活性σ因子的亲和力丧失。该复合物在沉降值上经历了两个阶段的变化,从营养生长阶段的14.5S到芽孢形成早期的13S种类,再到后期的16S种类。通过区带离心法研究了两个SpoO突变体。一个菌株是耐利福平(RfmR)突变体,未显示转录复合物有任何变化,而另一个Rfms菌株在t0之后转录复合物发生了部分演变。