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在无缺陷腺病毒 - 猴病毒40杂交病毒Ad2 + ND 1和Ad2 + ND 3的猴病毒40 DNA片段内，大肠杆菌RNA聚合酶启动RNA转录的首选位点。

Preferred site for initiation of RNA transcription by Escherichia coli RNA polymerase within the simian virus 40 DNA segment of the nondefective adenovirus-simian virus 40 hybrid viruses Ad2 + ND 1 and Ad2 + ND 3 .

作者信息

Zain B S, Dhar R, Weissman S M, Lebowitz P, Lewis A M

出版信息

J Virol. 1973 May;11(5):682-93. doi: 10.1128/JVI.11.5.682-693.1973.

DOI:10.1128/JVI.11.5.682-693.1973
PMID:4350713
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC355164/
Abstract

The DNA of simian virus 40 (SV40) was transcribed into RNA by Escherichia coli RNA polymerase at 18 to 24 C after synchronization of the initiation of RNA synthesis. After a brief synthetic period the RNA product contained relatively large amounts of sequences derived from a limited segment of SV40 DNA. The source for this pulse-labeled RNA was found to be a portion of the segment of SV40 DNA included within the nondefective adenovirus (Ad)-SV40 hybrid viruses, Ad2(+)ND(1) and Ad2(+)ND(3). After synthesis with [gamma-(32)P] ATP, Ad2(+)ND(1) and Ad2(+)ND(3) DNA transcripts contained an initial sequence missing from Ad2 transcripts. This sequence was identified as an initiation sequence for polymerase transcription of the SV40 DNA. Thus, there is a preferred site for initiation of in vitro transcription on the segment of SV40 DNA common to the nondefective Ad2(+)ND(1) and Ad2(+)ND(3) hybrid viruses.

摘要

在RNA合成起始同步化后，猿猴病毒40（SV40）的DNA在18至24摄氏度下被大肠杆菌RNA聚合酶转录为RNA。经过短暂的合成期后，RNA产物包含相对大量源自SV40 DNA有限片段的序列。发现这种脉冲标记RNA的来源是无缺陷腺病毒（Ad）-SV40杂交病毒Ad2(+)ND(1)和Ad2(+)ND(3)中包含的SV40 DNA片段的一部分。在用[γ-(32)P]ATP合成后，Ad2(+)ND(1)和Ad2(+)ND(3) DNA转录本包含Ad2转录本中缺失的初始序列。该序列被鉴定为SV40 DNA聚合酶转录的起始序列。因此，在无缺陷的Ad2(+)ND(1)和Ad2(+)ND(3)杂交病毒共有的SV40 DNA片段上存在体外转录起始的优选位点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baf0/355164/fc3d4483d182/jvirol00257-0085-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baf0/355164/002b1e1e6342/jvirol00257-0078-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baf0/355164/a2ae36a422f7/jvirol00257-0079-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baf0/355164/eb051319cd3c/jvirol00257-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baf0/355164/acc069f439c6/jvirol00257-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baf0/355164/b3a7197d31ba/jvirol00257-0083-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baf0/355164/a18d56fe3eee/jvirol00257-0084-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baf0/355164/fc3d4483d182/jvirol00257-0085-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baf0/355164/002b1e1e6342/jvirol00257-0078-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baf0/355164/a2ae36a422f7/jvirol00257-0079-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baf0/355164/eb051319cd3c/jvirol00257-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baf0/355164/acc069f439c6/jvirol00257-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baf0/355164/b3a7197d31ba/jvirol00257-0083-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baf0/355164/a18d56fe3eee/jvirol00257-0084-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baf0/355164/fc3d4483d182/jvirol00257-0085-a.jpg

相似文献

1
Preferred site for initiation of RNA transcription by Escherichia coli RNA polymerase within the simian virus 40 DNA segment of the nondefective adenovirus-simian virus 40 hybrid viruses Ad2 + ND 1 and Ad2 + ND 3 .在无缺陷腺病毒 - 猴病毒40杂交病毒Ad2 + ND 1和Ad2 + ND 3的猴病毒40 DNA片段内，大肠杆菌RNA聚合酶启动RNA转录的首选位点。
J Virol. 1973 May;11(5):682-93. doi: 10.1128/JVI.11.5.682-693.1973.
2
Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. VII. Characterization of the simian virus 40 RNA species induced by five nondefective hybrid viruses.无缺陷腺病毒2-猴病毒40杂交病毒的研究。VII. 五种无缺陷杂交病毒诱导的猴病毒40 RNA种类的特性
J Virol. 1973 May;11(5):672-81. doi: 10.1128/JVI.11.5.672-681.1973.
3
Control of simian virus 40 gene expression in adenovirus-simian virus 40 hybrid viruses. Synthesis of hybrid adenovirus 2-simian virus 40 RNA molecules in cells infected with a nondefective adenovirus 2-simian virus 40 hybrid virus.腺病毒 - 猴病毒40杂交病毒中猴病毒40基因表达的调控。在感染无缺陷腺病毒2 - 猴病毒40杂交病毒的细胞中杂交腺病毒2 - 猴病毒40 RNA分子的合成。
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4
Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. IV. Characterization of the simian virus 40 ribonucleic acid species induced by wild-type simian virus 40 and by the nondefective hybrid virus, Ad2 + ND 1 .非缺陷型腺病毒2-猴病毒40杂交病毒的研究。IV. 野生型猴病毒40和非缺陷型杂交病毒Ad2+ND1诱导的猴病毒40核糖核酸种类的特性
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Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. VI. Characterization of the DNA from five nondefective hybrid viruses.无缺陷腺病毒2-猴病毒40杂交病毒的研究。VI. 五种无缺陷杂交病毒DNA的特性分析
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Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. II. Relationship of adenovirus 2 deoxyribonucleic acid and simian virus 40 deoxyribonucleic acid in the Ad2+ND genome.无缺陷腺病毒2-猴病毒40杂交病毒的研究。II. 腺病毒2脱氧核糖核酸与猴病毒40脱氧核糖核酸在Ad2+ND基因组中的关系。
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Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. V. Isolation of additional hybrids which differ in their simian virus 40-specific biological properties.无缺陷腺病毒2-猴病毒40杂交病毒的研究。V. 分离出在猴病毒40特异性生物学特性上存在差异的其他杂交病毒。
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引用本文的文献

1
The mapping and ordering of fragments of SV40 DNA produced by restriction endonucleases.由限制性核酸内切酶产生的SV40 DNA片段的图谱绘制与排序。
Nucleic Acids Res. 1974 Jun;1(6):727-52. doi: 10.1093/nar/1.6.727.
2
The nucleotide sequence preceding an RNA polymerase initiation site on SV40 DNA. Part 2. The sequence of the early strand transcript.猴病毒40（SV40）DNA上RNA聚合酶起始位点之前的核苷酸序列。第2部分。早期链转录本的序列。
Nucleic Acids Res. 1974 Apr;1(4):595-611. doi: 10.1093/nar/1.4.595.
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The nucleotide sequence preceding an RNA polymerase initiation site on SV40 DNA. Part 1. The sequence of the late strand transcript.

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Nucleotide sequences of RNA transcribed in infected cells and by Escherichia coli RNA polymerase from a segment of simian virus 40 DNA.在受感染细胞中以及由大肠杆菌RNA聚合酶从猴病毒40 DNA的一个片段转录的RNA的核苷酸序列。
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A colinear map relating the simian virus 40 (SV40) DNA segments of six adenovirus-SV40 hybrids to the DNA fragments produced by restriction endonuclease cleavage of SV40 DNA.一种共线性图谱，它将六种腺病毒 - 猴病毒40（SV40）杂交体的SV40 DNA片段与通过SV40 DNA限制性内切酶切割产生的DNA片段联系起来。
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Nucleotide sequence of a ribonucleic acid transcribed in vitro from lambda phage deoxyribonucleic acid.从λ噬菌体脱氧核糖核酸体外转录的核糖核酸的核苷酸序列。
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Chromatography of 32P-labelled oligonucleotides on thin layers of DEAE-cellulose.32P 标记的寡核苷酸在二乙氨基乙基纤维素薄层上的色谱分析。
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