小牛卫星DNA I的一种可能结构。
A possible structure for calf satellite DNA I.
作者信息
Roizes G
出版信息
Nucleic Acids Res. 1976 Oct;3(10):2677-96. doi: 10.1093/nar/3.10.2677.
Abstract
Calf satellite DNA I (p = 1.715) has been hydrolysed by a number or restriction endonucleases. It consists of a repeating unit of 1460 nucleotide pairs within which the sites of Eco R II Mbo I, Sac I, Alu I, Ava II and Hha I were localised in comparison with those of Eco R I and Hind II. The distribution of the Hpa II, Sac I, Hha I, Hinf I and Mbo II sites within calf satellite DNA I, as well as that of several restriction endonuclease sites within calf satellite DNA III (p = 1.705) allowed me to define subsatellite fractions. Furthermore, some of the sites of the CpG containing restriction enzymes Hpa II and Hha I are lacking. The possible implications of these results are discussed.
摘要
小牛卫星DNA I（p = 1.715）已被多种限制性内切酶水解。它由一个1460个核苷酸对的重复单元组成，与Eco R I和Hind II的位点相比，Eco R II、Mbo I、Sac I、Alu I、Ava II和Hha I的位点定位在该重复单元内。小牛卫星DNA I中Hpa II、Sac I、Hha I、Hinf I和Mbo II位点的分布，以及小牛卫星DNA III（p = 1.705）中几个限制性内切酶位点的分布，使我能够定义亚卫星片段。此外，含CpG的限制性酶Hpa II和Hha I的一些位点缺失。讨论了这些结果可能的含义。
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