Li Q, Von Lanken C, Yang J, Lawrence C B, Hunt A G
Tobacco and Health Research Institute, University of Kentucky, Lexington 40546-0091, USA.
Plant Mol Biol. 2000 Jan;42(2):335-44. doi: 10.1023/a:1006354010997.
We have expressed the gene (PAB1) encoding the yeast polyadenylate-binding protein (Pab1p) in tobacco. Plants that accumulate the Pab1p display a range of abnormalities, ranging from a characteristic chlorosis in leaves to a necrosis and large inhibition of growth. The severity of these abnormalities reflects the levels of yeast Pab1p expression in the transgenic plants. In contrast, no obvious differences could be seen in callus cultures between the transgene and vector control. Plants that display PAB-associated abnormalities were resistant to a range of plant pathogens, and had elevated levels of expression of a pathogenesis-related gene. These two properties--impairment of growth and induction of defense responses--indicate that the yeast PAB1 gene can act as a disease lesion mimic gene in plants.
我们已在烟草中表达了编码酵母聚腺苷酸结合蛋白（Pab1p）的基因（PAB1）。积累Pab1p的植物表现出一系列异常，从叶片特有的黄化到坏死以及生长受到极大抑制。这些异常的严重程度反映了转基因植物中酵母Pab1p的表达水平。相比之下，在转基因和载体对照的愈伤组织培养物中未观察到明显差异。表现出与PAB相关异常的植物对多种植物病原体具有抗性，并且病程相关基因的表达水平有所升高。这两个特性——生长受损和防御反应诱导——表明酵母PAB1基因可作为植物中的疾病模拟基因。
