Ogbadoyi E, Ersfeld K, Robinson D, Sherwin T, Gull K
School of Biological Sciences, University of Manchester, UK.
Chromosoma. 2000 Mar;108(8):501-13. doi: 10.1007/s004120050402.
The structural basis of mitosis, spindle organisation and chromosome segregation, in the unicellular parasite Trypanosoma brucei is poorly understood. Here, using immunocytochemistry, fluorescent in situ hybridisation and electron microscopy, we provide a detailed analysis of mitosis in this parasite. We describe the organisation of the mitotic spindle during different stages of mitosis, the complex ultrastructure of kinetochores and the identification of a potential spindle-organising centre in the mitotic nucleus. We investigate the dynamics of chromosome segregation using telomeric and chromosome-specific probes. We also discuss the problems involved in chromosome segregation in the light of the fact that the T. brucei karyotype has 22 chromosomes in the apparent presence of only eight ultrastructurally defined kinetochores.
对于单细胞寄生虫布氏锥虫有丝分裂的结构基础、纺锤体组织和染色体分离，人们了解甚少。在此，我们运用免疫细胞化学、荧光原位杂交和电子显微镜技术，对该寄生虫的有丝分裂进行了详细分析。我们描述了有丝分裂不同阶段有丝分裂纺锤体的组织情况、动粒的复杂超微结构以及在有丝分裂细胞核中潜在纺锤体组织中心的鉴定。我们使用端粒和染色体特异性探针研究了染色体分离的动态过程。鉴于布氏锥虫的核型有22条染色体，而明显仅存在8个超微结构确定的动粒，我们还讨论了染色体分离中涉及的问题。
