Conger B V
Int J Radiat Biol Relat Stud Phys Chem Med. 1975 Mar;27(3):271-81. doi: 10.1080/09553007514551561.
This investigation was conducted to determine the maximum r.b.e. of fission neutrons for the induction of yellow-green sectors in maize. Seeds heterozygous for the yg2 locus were adjusted to 13 per cent water content, irradiated with varying doses for eitherfission neutrons or 60Co gamma-radiation, and soaked for 2 hours in nitrogen-bubbled water at ambient temperature before planting. Frequency of yg2 sectors, as scored in leaves 4 and 5, increase linearly for both radiations. The r.b.e. values were greater than1100 at 0.40 sectors per leaf 4 and greater than 600 at 0.20 sectors per leaf 5; however, at these low frequencies, the standard errors were 3 times greater than the r.b.e. At 6.0 sectors per leaf 4, the r.b.e. values were 111 plus or minus 9; and at 3.0sectors per leaf 5, 118 plus or minus 21. As measured by sectors per krad, mutagenic efficiency increased with increased dose of gamma-radiation; the opposite was true for fission neutrons. This trend was more evident for leaf 4 than for leaf 5.
本研究旨在确定裂变中子诱发玉米黄绿扇形区的最大相对生物效应（r.b.e.）。将yg2位点杂合的种子含水量调整到13%，用不同剂量的裂变中子或60Coγ射线进行辐照，并在种植前于室温下在通氮水中浸泡2小时。在第4片和第5片叶子中统计的yg2扇形区频率，两种辐射均呈线性增加。在第4片叶子每叶0.40个扇形区时，r.b.e.值大于1100；在第5片叶子每叶0.20个扇形区时，r.b.e.值大于600；然而，在这些低频率下，标准误差是r.b.e.的3倍。在第4片叶子每叶6.0个扇形区时，r.b.e.值为111±9；在第5片叶子每叶3.0个扇形区时，r.b.e.值为118±21。以每千拉德的扇形区数衡量，诱变效率随γ射线剂量增加而提高；裂变中子则相反。这种趋势在第4片叶子上比在第5片叶子上更明显。
