Mackey L, Jarrett W, Jarrett O, Wilson L
J Natl Cancer Inst. 1975 Jun;54(6):1483-7. doi: 10.1093/jnci/54.6.1483.
The mixed antiglobulin reaction and the formation of nonimmune rosettes with guinea-pig red blood cells (RBC) distinguished feline B and T cells, respectively. In a cat with thymic lymphosarcoma, the cells reacting in these tests formed separate, nonoverlapping populations. The malignant cells were large lymphoblasts replacing the normal thymus and infiltrating local lymph nodes, where they localized only in the paracortical, i.e., thymus-dependent areas. Cells from the nodes could therefore be identified as malignant or normal by their size. The mixed antiglobulin reaction showed that the malignant cells did not carry the surface Ig characteristic of B cells, whereas these malignant cells formed nonimmune rosettes with guinea-pig RBC. Among lymph node cells, most surviving normal and small lymphocytes, from outside the thymus-dependent areas, reacted as B cells. The morphologic evidence therefore corroborated the test results, which indicated that the formation of rosettes with guinea-pig RBC seems a reliable means for the demonstration of T cells in the cat.
混合抗球蛋白反应以及与豚鼠红细胞（RBC）形成非免疫性玫瑰花结分别区分了猫的B细胞和T细胞。在一只患有胸腺淋巴肉瘤的猫中，在这些试验中发生反应的细胞形成了各自独立、互不重叠的群体。恶性细胞是大淋巴细胞，取代了正常胸腺并浸润局部淋巴结，它们仅定位在副皮质区，即胸腺依赖区。因此，淋巴结中的细胞可以根据其大小被鉴定为恶性或正常。混合抗球蛋白反应表明，恶性细胞不具有B细胞特征性的表面免疫球蛋白，而这些恶性细胞与豚鼠红细胞形成了非免疫性玫瑰花结。在淋巴结细胞中，大多数来自胸腺依赖区以外的存活正常小淋巴细胞表现为B细胞反应。形态学证据因此证实了试验结果，这表明与豚鼠红细胞形成玫瑰花结似乎是在猫中证明T细胞的可靠方法。
