青蛙神经肌肉接头中药物诱导电导的电压依赖性。
Voltage-dependence of drug-induced conductance in frog neuromuscular junction.
作者信息
Neher E, Sakmann B
出版信息
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2140-4. doi: 10.1073/pnas.72.6.2140.
Abstract
Membrane currents from voltage-clamped frog muscle fibers were recorded during iontophoretic application of steady doses of carbachol, acetylcholine, and suberyldicholine to the endplate region. In the presence of these drugs, an exponentially relaxing current was observed after step changes of membrane potential. The time constant of relaxation was found to be voltage-dependent. It was equal to the time constant obtained from the autocorrelation function of drug-induced conductance fluctuations measured under similar conditions. Analysis of instantaneous current at the on- and offsets of voltageclamp pulses showed that there is no shift in equilibrium potential during the pulses.
摘要
在向终板区域离子电渗施加稳定剂量的卡巴胆碱、乙酰胆碱和辛二酰胆碱期间，记录了电压钳制的青蛙肌肉纤维的膜电流。在这些药物存在的情况下，膜电位阶跃变化后观察到指数衰减电流。发现弛豫时间常数与电压有关。它等于在类似条件下测量的药物诱导的电导波动的自相关函数所获得的时间常数。对电压钳制脉冲开启和关闭时的瞬时电流分析表明，脉冲期间平衡电位没有偏移。
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