使用浴加辛二酰胆碱进行的松弛实验。
Relaxation experiments using bath-applied suberyldicholine.
作者信息
Adams P R
出版信息
J Physiol. 1977 Jun;268(2):271-89. doi: 10.1113/jphysiol.1977.sp011857.
Abstract
- The effect of step changes in membrane potential on the end-plate conductance change produced by bath-applied suberyldicholine was studied in voltage-clamped frog muscle fibres. 2. The suberyldicholine-induced conductance increased exponentially from its previous equilibrium level to a new equilibrium level following a step hyperpolarization. 3. For low suberyldicholine concentrations the time constant of this relaxation was independent of the concentration. 4. For low suberyldicholine concentrations the voltage dependence of equilibrium conductance and relaxation time constants was identical. 5. Bungarotoxin pretreatment did not affect the responses beyond a simple reduction in their amplitude. 6. The conductance evoked by high suberyldicholine concentrations was less voltage-sensitive than that evoked by low concentrations. 7. A new model for explaining noise and relaxation data is proposed. This postulates rate-limiting binding steps followed by a voltage-dependent isomerization.
摘要
- 在电压钳制的青蛙肌肉纤维中，研究了膜电位的阶跃变化对浴加辛二酰胆碱所产生的终板电导变化的影响。2. 阶跃超极化后，辛二酰胆碱诱导的电导从其先前的平衡水平呈指数增加至新的平衡水平。3. 对于低浓度的辛二酰胆碱，这种松弛的时间常数与浓度无关。4. 对于低浓度的辛二酰胆碱，平衡电导和松弛时间常数的电压依赖性是相同的。5. 筒箭毒碱预处理除了简单地降低反应幅度外，并不影响反应。6. 高浓度辛二酰胆碱诱发的电导比低浓度诱发的电导对电压的敏感性更低。7. 提出了一个用于解释噪声和松弛数据的新模型。该模型假定存在限速结合步骤，随后是电压依赖性异构化。
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