Fargette F, Grelet N, Rapoport G
Biochimie. 1979;61(1):93-100. doi: 10.1016/s0300-9084(79)80317-x.
The nature of tetracycline resistance was studied in a strain of Bacillus subtilis carrying the plasmid pT 127 in comparison with the parental strain. The resistance has been shown to be inducible in both strains upon exposure to subinhibitory concentrations of tetracycline. No modification of the protein-synthesizing activity of the ribosomes or intracellular inactivation of the antibiotic was observed in both strains. Accumulation of labeled tetracycline in B. subtilis was found to be particularly low in the wild-type strain, compared to other bacterial species, with concentration gradients of only 2 to 3 fold. From the kinetics obtained it is likely that the permeation of the antibiotic does not correspond to an active process in B. subtilis. A fairly good correlation was established between the level of resistance obtained after induction or by the presence of the plasmid pT 127 and a decrease in the binding capacity of the cell for the antibiotic.
对携带质粒pT 127的枯草芽孢杆菌菌株与亲代菌株相比的四环素抗性性质进行了研究。已表明，在暴露于亚抑制浓度的四环素时，两种菌株中的抗性均可诱导产生。在两种菌株中均未观察到核糖体蛋白质合成活性的改变或抗生素的细胞内失活。与其他细菌物种相比，发现野生型菌株中标记的四环素在枯草芽孢杆菌中的积累特别低，浓度梯度仅为2至3倍。从获得的动力学结果来看，抗生素在枯草芽孢杆菌中的渗透可能与一个主动过程不符。诱导后或通过质粒pT 127的存在获得的抗性水平与细胞对抗生素的结合能力降低之间建立了相当好的相关性。
