枯草芽孢杆菌中的DNA克隆
DNA cloning in Bacillus subtilis.
作者信息
Ehrlich S D
出版信息
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1433-6. doi: 10.1073/pnas.75.3.1433.
Abstract
A plasmid pC194, encoding resistance to chloramphenicol, can serve as a cloning vector in Bacillus subtilis 168 for other HindIII-cleaved DNA segments. Replicons constructed by linking pC194 to several Escherichia coli plasmids can be used to introduce and compare the expression of the same genes in these two bacterial hosts.
摘要
编码氯霉素抗性的质粒pC194可作为枯草芽孢杆菌168中其他经HindIII切割的DNA片段的克隆载体。通过将pC194与几种大肠杆菌质粒连接构建的复制子可用于在这两种细菌宿主中导入和比较相同基因的表达。
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