Yokota M, Tsuda I, Tatsumi N
Department of Clinical and Laboratory Medicine, Osaka City University Medical School, Abeno, Osaka, Japan.
J Clin Lab Anal. 2000;14(3):97-100. doi: 10.1002/(SICI)1098-2825(2000)14:3<97::AID-JCLA3>3.0.CO;2-F.
The availability of Nagarse, a protease, as a substitute for proteinase K for digestion of leukocytic or bacterial DNAs was studied. The amount and purity of DNAs extracted from leukocytes and several bacterial strains with Nagarse were compared with those of DNAs treated with proteinase. Nagarse exhibited the same behavior as proteinase K in digesting leukocytes, and it could also be used for bacterial digestion for physical mapping of genomic DNA by biased sinusoid field gel electrophoresis. Nagarse was thus comparable to proteinase K for use in biochemical experiments. The principal advantage of Nagarse is that it is inexpensive, unlike proteinase K, and our findings indicated that Nagarse is very useful as a substitute for proteinase K for the DNA study.
研究了一种蛋白酶Nagarse作为蛋白酶K的替代品用于消化白细胞或细菌DNA的可行性。将用Nagarse从白细胞和几种细菌菌株中提取的DNA的量和纯度与用蛋白酶处理的DNA的量和纯度进行了比较。Nagarse在消化白细胞方面表现出与蛋白酶K相同的特性，并且它还可用于通过交变正弦电场凝胶电泳对基因组DNA进行物理图谱分析的细菌消化。因此，在生化实验中，Nagarse与蛋白酶K相当。Nagarse的主要优点是它不像蛋白酶K那样昂贵，我们的研究结果表明，Nagarse作为蛋白酶K的替代品用于DNA研究非常有用。
