Liu Xiaoping, Yin Feifei, Zhu Zheng, Hou Dianhai, Wang Jun, Zhang Lei, Wang Manli, Wang Hualin, Hu Zhihong, Deng Fei
State Key Laboratory of Virology, Virus Resource and Bioinformatics Center, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, China.
PLoS One. 2014 Oct 17;9(10):e110023. doi: 10.1371/journal.pone.0110023. eCollection 2014.
The complete nucleotide sequence of Sucra jujuba nucleopolyhedrovirus (SujuNPV) was determined by 454 pyrosequencing. The SujuNPV genome was 135,952 bp in length with an A+T content of 61.34%. It contained 131 putative open reading frames (ORFs) covering 87.9% of the genome. Among these ORFs, 37 were conserved in all baculovirus genomes that have been completely sequenced, 24 were conserved in lepidopteran baculoviruses, 65 were found in other baculoviruses, and 5 were unique to the SujuNPV genome. Seven homologous regions (hrs) were identified in the SujuNPV genome. SujuNPV contained several genes that were duplicated or copied multiple times: two copies of helicase, DNA binding protein gene (dbp), p26 and cg30, three copies of the inhibitor of the apoptosis gene (iap), and four copies of the baculovirus repeated ORF (bro). Phylogenetic analysis suggested that SujuNPV belongs to a subclade of group II alphabaculovirus, which differs from other baculoviruses in that all nine members of this subclade contain a second copy of dbp.
采用454焦磷酸测序法测定了枣尺蠖核型多角体病毒（SujuNPV）的全核苷酸序列。SujuNPV基因组长度为135,952 bp，A+T含量为61.34%。它包含131个推定的开放阅读框（ORF），覆盖基因组的87.9%。在这些ORF中，37个在所有已完成全序列测定的杆状病毒基因组中保守，24个在鳞翅目杆状病毒中保守，65个在其他杆状病毒中发现，5个是SujuNPV基因组特有的。在SujuNPV基因组中鉴定出7个同源区域（hrs）。SujuNPV包含几个重复或多次复制的基因：解旋酶、DNA结合蛋白基因（dbp）、p26和cg30各有两个拷贝，凋亡抑制基因（iap）有三个拷贝，杆状病毒重复ORF（bro）有四个拷贝。系统发育分析表明，SujuNPV属于II组甲型杆状病毒的一个亚分支，该亚分支与其他杆状病毒的不同之处在于，该亚分支的所有九个成员都含有第二个dbp拷贝。
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