Hall W, Ogden G R, Saleh A H, Hopwood D, Ross P E
Department of Molecular & Cellular Pathology, Ninewells Hospital & Medical School, University of Dundee, Scotland.
J Oral Pathol Med. 2000 May;29(5):220-5. doi: 10.1034/j.1600-0714.2000.290505.x.
The structure of the oral mucosa has been extensively studied but its cell physiology has been less well characterised. This study aimed to show the range in variation in fluid phase endocytic capability in biopsies from different oral sites. Oral epithelial cells were obtained from both biopsies and single-cell suspensions obtained by brushing the oral cavity. Biopsies in organ culture and single cells in suspension were incubated with fluorescent microspheres of 0.02, 0.1 or 1.0 microm diameter. Endocytosis of fluorescent microspheres was quantitated by flow cytometry and visualised by confocal microscopy. Epithelial cells from all oral sites that were sampled internalised 0.02 microm and 0.1 microm but not 1.0 microm microspheres, with no significant differences observed between oral regions. Single cells from non-cancer patients endocytosed significantly more 0.02 microm microspheres than cells removed from patients with oral cancer. This model may be used to study integrated oral cell function both in health and disease.
口腔黏膜的结构已得到广泛研究，但其细胞生理学特征尚不明确。本研究旨在展示不同口腔部位活检组织液相内吞能力的变化范围。口腔上皮细胞取自活检组织以及通过口腔刷取获得的单细胞悬液。将器官培养中的活检组织和悬浮的单细胞与直径为0.02、0.1或1.0微米的荧光微球一起孵育。通过流式细胞术对荧光微球的内吞作用进行定量，并通过共聚焦显微镜进行可视化观察。所有取样口腔部位的上皮细胞均可内化0.02微米和0.1微米的微球，但不能内化1.0微米的微球，不同口腔区域之间未观察到显著差异。非癌症患者的单细胞内化0.02微米微球的能力明显高于口腔癌患者的细胞。该模型可用于研究健康和疾病状态下口腔细胞的综合功能。
