膜钠泵活性对人外周血淋巴细胞与绵羊红细胞形成早期玫瑰花结能力的影响。

The influence of the membrane sodium pump activity on the ability of human peripheral blood lymphocytes to form early rosettes with SRBC.

作者信息

Witkowski J

出版信息

Immunology. 1985 Jun;55(2):339-45.

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1453614/

Abstract

The influence of changes of the cation-transporting activity of the membrane Na,K ATP-ase of human peripheral blood lymphocytes on their ability to form 'early' and 'late' rosettes with SRBC was investigated. It was shown that inhibition of the Na,K ATP-ase-dependent cation transport through the lymphocyte membrane by means of incubation of the cells with various specific inhibitors of the enzyme (ouabain, vanadate) or depletion of the magnesium cations from the incubation medium caused the rise in the percentage of early rosettes, having no influence on the late E rosette percentage. The possible explanation of the observed phenomena is discussed with regard to (ion transport-dependent) electrical forces present at the surface of resting and activated lymphocytes.

摘要

研究了人外周血淋巴细胞膜Na，K ATP酶阳离子转运活性的变化对其与SRBC形成“早期”和“晚期”玫瑰花结能力的影响。结果表明，通过用该酶的各种特异性抑制剂（哇巴因、钒酸盐）孵育细胞或从孵育培养基中耗尽镁阳离子来抑制依赖Na，K ATP酶的阳离子通过淋巴细胞膜的转运，会导致早期玫瑰花结百分比升高，而对晚期E玫瑰花结百分比没有影响。针对静息和活化淋巴细胞表面存在的（依赖离子转运的）电力量，讨论了观察到的现象的可能解释。

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