Smith R, Kerman R, Ezdinli E, Stefani S
J Immunol Methods. 1975;8(1-2):175-84. doi: 10.1016/0022-1759(75)90094-0.
A modified procedure is described for the detection of a sub-population of peripheral blood T-cells in normal human adults. This sub-population of T-cells has been called the active rosette forming cell by others. The advantages of this modified procedure compared with that previously described are: 1) the elimination of 60 mon incubation period for lymphocytes prior to the assay and 2) the elimination of the need for fetal calf serum in the assay. The mean percentage of RFC in the peripheral blood of 80 healthy adult controls using the modified procedure is 25.2+/-5.5% in buffer lacking FCS. The results were highly reproducible and comparable to the results obtained using the procedure previously described. It was concluded that a 60 min pre-incubation of lymphocytes and the use of FCS are not necessary when the appropriate SRBS: lymphocyte ratio is used in the determination of human adult active RFC. The use of this procedure in evaluating the immune competence of cancer patients is discussed.
本文描述了一种用于检测正常成年人外周血T细胞亚群的改良方法。该T细胞亚群被其他人称为活性花环形成细胞。与先前描述的方法相比，这种改良方法的优点是：1）在检测前消除了淋巴细胞60分钟的孵育期；2）在检测中无需使用胎牛血清。使用改良方法，在不含胎牛血清的缓冲液中，80名健康成人对照组外周血中RFC的平均百分比为25.2±5.5%。结果具有高度可重复性，且与使用先前描述的方法获得的结果相当。得出的结论是，当在测定成人活性RFC时使用适当的SRBC：淋巴细胞比例时，淋巴细胞60分钟的预孵育和胎牛血清的使用并非必要。本文还讨论了该方法在评估癌症患者免疫能力方面的应用。
