Quan P C, Burtin P
Br J Cancer. 1978 Nov;38(5):606-11. doi: 10.1038/bjc.1978.256.
High-affinity rosette-forming T-cell assays were performed by incubation of peripheral-blood mononuclear cells with sheep erythrocytes (E) at 29 degrees C. As compared with normal controls, the levels of high-affinity rosette-forming cells (RFC) were much more frequently depressed in cancer patients than were the total E-RFC incubated at 4 degrees C. Only 2/83 normal controls had less than 38% 29 degrees C E-RFC (mean 48 +/- 5), whilst 78/89 cancer patients were below this level. The few postoperative patients tested exhibited a normal range of 29 degrees C E-RFC. The 29 degrees C E-rosette assay gives reproducible counts of a T-cell subset, and is a sensitive assay for evaluating the immune status of cancer patients.
高亲和力玫瑰花结形成T细胞检测是通过在29摄氏度下将外周血单个核细胞与绵羊红细胞(E)孵育来进行的。与正常对照组相比,癌症患者中高亲和力玫瑰花结形成细胞(RFC)的水平比在4摄氏度下孵育的总E-RFC更频繁地降低。只有2/83的正常对照者29摄氏度E-RFC低于38%(平均48±5),而89例癌症患者中有78例低于此水平。少数接受检测的术后患者29摄氏度E-RFC处于正常范围。29摄氏度E玫瑰花结检测可对T细胞亚群进行可重复计数,是评估癌症患者免疫状态的一种敏感检测方法。