拟南芥5S核糖体DNA阵列分析：物理图谱绘制与染色体特异性多态性
Analysis of 5S rDNA arrays in Arabidopsis thaliana: physical mapping and chromosome-specific polymorphisms.
作者信息
Cloix C, Tutois S, Mathieu O, Cuvillier C, Espagnol M C, Picard G, Tourmente S
机构信息
Unité Mixte de Recherche, 6547 BIOMOVE, Université Blaise Pascal, 63177 Aubière Cedex, France.
出版信息
Genome Res. 2000 May;10(5):679-90. doi: 10.1101/gr.10.5.679.
Abstract
A physical map of a pericentromeric region of chromosome 5 containing a 5S rDNA locus and spanning approximately 1000 kb was established using the CIC YAC clones. Three 5S rDNA arrays were resolved in this YAC contig by PFGE analysis and we have mapped different types of sequences between these three blocks. 5S rDNA units from each of these three arrays of chromosome 5, and from chromosomes 3 and 4, were isolated by PCR. A total of 38 new DNA sequences were obtained. Two types of 5S rDNA repeated units exist: the major variant with 0.5-kb repeats and one with short repeats (251 bp) only detected on YAC 11A3 from chromosome 3. Although the 38 sequences displayed noticeable heterogeneity, we were able to group them according to their 5S array origin. The presence of 5S array-specific variants was confirmed with the restriction polymorphism study of all the YACs carrying 5S units.
摘要
利用CIC酵母人工染色体（YAC）克隆构建了5号染色体着丝粒周围区域的物理图谱，该区域包含一个5S核糖体DNA（rDNA）位点，跨度约为1000千碱基对（kb）。通过脉冲场凝胶电泳（PFGE）分析，在这个YAC重叠群中解析出三个5S rDNA阵列，并且我们已经在这三个区段之间绘制了不同类型的序列图谱。通过聚合酶链反应（PCR）从5号染色体的这三个阵列以及3号和4号染色体中分离出5S rDNA单元。总共获得了38个新的DNA序列。存在两种类型的5S rDNA重复单元：主要变体具有0.5 kb的重复序列，另一种具有短重复序列（251 bp），仅在3号染色体的YAC 11A3上检测到。尽管这38个序列表现出明显的异质性，但我们能够根据它们的5S阵列来源对其进行分组。通过对所有携带5S单元的YAC进行限制性多态性研究，证实了5S阵列特异性变体的存在。
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