Tutois S, Cloix C, Cuvillier C, Espagnol M C, Lafleuriel J, Picard G, Tourmente S
U.M.R. 6547 BIOMOVE, Université Blaise Pascal, Aubière, France.
Chromosome Res. 1999;7(2):143-56. doi: 10.1023/a:1009211603248.
The Arabidopsis thaliana CIC YAC 2D2, 510 kb long and containing a small block of 180 bp satellite units was subcloned after EcoR1 digestion in the pBluescript plasmid. One of these clones was mapped genetically in the pericentromeric region of chromosome 5. The analysis of 40 subclones of this YAC showed that they all contain repeated sequences with a high proportion of transposable elements. Three new retrotransposons, two Ty-3 Gypsy-like and one Ty-1 Copia, were identified in addition to two new tandem-repeat families. A physical map of the chromosome 5 pericentromeric region was established using CIC YAC clones, spanning around 1000 kb. This contig extends from the CIC YAC 9F5 and 7A2 positioned on the left arm of chromosome 5 to a 5S rDNA genes block localized by in-situ hybridization in the pericentromeric region. Hybridization of the subclones on the CIC YAC library showed that some of them are restricted to the pericentromeric region of chromosome 5 and represent specific markers of this region.
拟南芥CIC YAC 2D2长510 kb，包含一小段180 bp的卫星单元，经EcoR1酶切后亚克隆到pBluescript质粒中。其中一个克隆在第5号染色体的着丝粒周围区域进行了遗传定位。对该YAC的40个亚克隆的分析表明，它们都含有重复序列，其中转座元件比例很高。除了两个新的串联重复家族外，还鉴定出三个新的逆转座子，两个类似Ty-3 Gypsy的和一个Ty-1 Copia。利用CIC YAC克隆构建了第5号染色体着丝粒周围区域的物理图谱，跨度约1000 kb。这个重叠群从位于第5号染色体左臂上的CIC YAC 9F5和7A2延伸到通过原位杂交定位在着丝粒周围区域的一个5S rDNA基因块。亚克隆在CIC YAC文库上的杂交表明，其中一些仅限于第5号染色体的着丝粒周围区域，是该区域的特异性标记。
