Indaram A V, Visvalingam V, Locke M, Bank S
Division of Gastroenterology, Long Island Jewish Medical Center, Albert Einstein College of Medicine, New Hyde Park, New York 11040, USA.
Am J Gastroenterol. 2000 May;95(5):1221-5. doi: 10.1111/j.1572-0241.2000.02013.x.
We measured the mucosal levels of interleukin 1beta (IL-1beta), IL-2, IL-6, and IL-8 in affected segments of radiation-induced proctosigmoiditis and compared these with the levels in normal controls and inflammatory bowel disease (IBD) patients.
Thirteen patients with histologically proven radiation proctosigmoiditis, 32 patients with active ulcerative colitis (UC), 35 patients with Crohn's disease, and 15 normal subjects undergoing routine colonoscopy were included in the study. All patients underwent colonoscopy and mucosal biopsies were obtained from both diseased and normal-appearing areas. Mucosal levels of IL-1beta, IL-2, IL-6, and IL-8 were determined by solid-phase enzyme-linked immunosorbent assay (ELISA) using the Quantikine method (R&D Systems, Minneapolis, MN). All the data were statistically analyzed using Student's t test.
The mucosal levels of IL-2, IL-6, and IL-8 were significantly higher in both diseased segments (5.62 +/- 0.13, 1.60 +/- 0.31, and 21.45 +/- 4.03 pg/ml, respectively) and normal-appearing segments (3.83 +/- 0.78, 1.36 +/- 0.34, and 13.45 +/- 3.18 pg/mg) in the radiation proctitis group compared to those of normal control subjects (1.74 +/- 0.23, 0.67 +/- 0.09, and 4.99 +/- 1.39 pg/mg). These differences were statistically significant (p < 0.05). In the UC group, IL-1beta, IL-6, and IL-8 levels in diseased segments were 4.98 +/- 0.53, 2.22 +/- 0.28, and 88.85 +/- 8.05 pg/mg, respectively. In Crohn's disease patients, IL-1beta, IL-6, and IL-8 levels were 5.45 +/- 0.93, 2.88 +/- 0.58, and 61.68 +/- 10.02 pg/mg, respectively. All these levels were significantly higher (p < 0.05) compared with IL-1beta, IL-6, and IL-8 levels from normal segments of IBD patients. Compared with the radiation proctitis patients, the levels of IL-6 and IL-8 were significantly higher in the IBD group.
The mucosal levels of IL-2, IL-6, and IL-8 were significantly higher in both diseased and normal segments of colon in patients with radiation proctitis, compared with normal controls. Only IL-1beta levels were significantly higher in diseased segments, compared with endoscopically normal-appearing segments in radiation proctitis. These results indicate that there is a similarity in the activation of mucosal cytokines between IBD and radiation proctosigmoiditis. This may partly explain the beneficial effects of similar topical and systemic agents such as steroids and mesalamine compounds when used in radiation-induced proctosigmoiditis.
我们测量了放射性直肠乙状结肠炎受累节段中白细胞介素1β（IL-1β）、IL-2、IL-6和IL-8的黏膜水平，并将其与正常对照者和炎症性肠病（IBD）患者的水平进行比较。
本研究纳入了13例经组织学证实的放射性直肠乙状结肠炎患者、32例活动期溃疡性结肠炎（UC）患者、35例克罗恩病患者以及15例接受常规结肠镜检查的正常受试者。所有患者均接受结肠镜检查，并从病变部位和外观正常的部位获取黏膜活检组织。采用Quantikine方法（R&D Systems，明尼阿波利斯，明尼苏达州）通过固相酶联免疫吸附测定（ELISA）法测定IL-1β、IL-2、IL-6和IL-8的黏膜水平。所有数据均采用学生t检验进行统计学分析。
与正常对照者（分别为1.74±0.23、0.67±0.09和4.99±1.39 pg/mg）相比，放射性直肠炎组病变节段（分别为5.62±0.13、1.60±0.31和21.45±4.03 pg/ml）和外观正常节段（分别为3.83±0.78、1.36±0.34和13.45±3.18 pg/mg）中IL-2、IL-6和IL-8的黏膜水平均显著升高。这些差异具有统计学意义（p<0.05）。在UC组中，病变节段的IL-1β、IL-6和IL-8水平分别为4.98±0.53、2.22±0.28和88.85±8.05 pg/mg。在克罗恩病患者中，IL-1β、IL-6和IL-8水平分别为5.45±0.93、2.88±0.58和61.68±10.02 pg/mg。与IBD患者正常节段的IL-1β、IL-6和IL-8水平相比，所有这些水平均显著升高（p<0.05）。与放射性直肠炎患者相比，IBD组中IL-6和IL-8水平显著更高。
与正常对照者相比，放射性直肠炎患者结肠病变节段和正常节段中IL-2、IL-6和IL-8的黏膜水平均显著升高。与放射性直肠炎内镜下外观正常的节段相比，仅病变节段的IL-1β水平显著升高。这些结果表明，IBD和放射性直肠乙状结肠炎之间在黏膜细胞因子激活方面存在相似性。这可能部分解释了类固醇和柳氮磺胺吡啶类化合物等类似的局部和全身药物在放射性直肠乙状结肠炎中使用时的有益效果。
