二硝基氯苯接触致敏和环磷酰胺处理豚鼠后的红细胞玫瑰花结（RRBC）和红细胞抗体补体玫瑰花结（EAC）形成
RRBC and EAC rosette formation after DNCB contact sensitization and cyclophosphamide treatment of guinea-pigs.
作者信息
Scheper R J, Baak J P, Hiisivara S, Veldhuizen R W
出版信息
Immunology. 1975 Nov;29(5):909-19.
Rabbit erythrocyte (RRBC) and EAC rosette formation, for detection of T and B lymphocytes respectively, was studied in thymus, lymph nodes and peripheral blood of guinea-pigs. Using two different methods for preparation of RRBC rosette-forming cells (RFC) it was found that, without an incubation period at 37 degrees, a smaller part of the pool of T cells is detected which is particularly sensitive to cyclophosphamide (cy). RRBC rosette formation was studied for 2 weeks after treatment with one high dose of cy and appeared to be minimal in the thymus after 1 week and maximal in the lymph nodes at about day 3. EAC rosette formation was completely blocked by cy treatment. Four days after contact sensitization with 2,4-dinitrochlorobenzene (DNCB) no percentual increase was found in RRBC-RFC, suggesting a parallel increase of B and T cells in stimulated lymph nodes. Cytological screening of the rosettes at day 4 after DNCB showed that of the RRBC-RFC approximately 17 per cent were blast cells, while no difference was detected in the number of blast cells participating in EAC rosette formation as compared to the unstimulated lymph nodes. In contrast to RRBC-RFC the percentage of EAC-RFC in draining lymph nodes is increased after contact sensitization. The mechanism of this increase is discussed.
分别用于检测T淋巴细胞和B淋巴细胞的兔红细胞（RRBC）和EAC玫瑰花结形成，在豚鼠的胸腺、淋巴结和外周血中进行了研究。使用两种不同的方法制备RRBC玫瑰花结形成细胞（RFC），发现如果没有在37℃的孵育期，只能检测到较小部分的T细胞池，而这部分T细胞对环磷酰胺（cy）特别敏感。在用一剂高剂量的cy治疗后，对RRBC玫瑰花结形成进行了2周的研究，结果显示，胸腺中的RRBC玫瑰花结形成在1周后降至最低，而在淋巴结中，大约在第3天达到最高。cy治疗完全阻断了EAC玫瑰花结的形成。在用2,4-二硝基氯苯（DNCB）进行接触致敏4天后，未发现RRBC-RFC有百分比增加，这表明在受刺激的淋巴结中B细胞和T细胞同时增加。在DNCB致敏后第4天对玫瑰花结进行细胞学筛查，结果显示，RRBC-RFC中约17%为母细胞，而与未受刺激的淋巴结相比，参与EAC玫瑰花结形成的母细胞数量没有差异。与RRBC-RFC相反，接触致敏后引流淋巴结中EAC-RFC的百分比增加。文中讨论了这种增加的机制。
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