Bullen J J, Armstrong J A
Immunology. 1979 Apr;36(4):781-91.
Rabbit polymorphonuclear leucocytes contain the iron-binding protein lactoferrin, and can rapidly phagocytose and destroy Pseudomonas aeruginosa. The lactoferrin normally has a large unsaturated iron-binding capacity. If the cells are exposed to a ferritin-antibody complex, large amounts of this are phagocytosed and appear in the cytoplasmic granules and phagosomes. This leads to saturation of the cellular iron-binding protein with Fe. In these circumstances, the bactericidal power of the cells is greatly reduced with the result that some phagocytosed bacteria survive and eventually grow and destroy the cells. An apoferritin-antibody complex used as a control is also phagocytosed but has no effect on the bactericidal power of the cell. The results support the view that lactoferrin plays an essential role in the bactericidal power of the cell.
兔多形核白细胞含有铁结合蛋白乳铁蛋白，并且能够快速吞噬和破坏铜绿假单胞菌。乳铁蛋白通常具有很大的不饱和铁结合能力。如果将细胞暴露于铁蛋白-抗体复合物中，大量该复合物会被吞噬并出现在细胞质颗粒和吞噬体中。这会导致细胞铁结合蛋白被铁饱和。在这些情况下，细胞的杀菌能力会大大降低，结果是一些被吞噬的细菌存活下来并最终生长并破坏细胞。用作对照的脱铁铁蛋白-抗体复合物也会被吞噬，但对细胞的杀菌能力没有影响。这些结果支持了乳铁蛋白在细胞杀菌能力中起重要作用的观点。
