短期暴露于低于治疗浓度的葡萄糖酸氯己定对口腔白色念珠菌芽管形成的影响及其与抗真菌后效应的关系。

The effect of brief exposure to sub-therapeutic concentrations of chlorhexidine gluconate on the germ tube formation of oral Candida albicans and its relationship to post-antifungal effect.

作者信息

Ellepola A N, Samaranayake L P

机构信息

Prince Philip Dental Hospital, Faculty of Dentistry, University of Hong Kong, Hong Kong SAR.

出版信息

Oral Dis. 2000 May;6(3):166-71. doi: 10.1111/j.1601-0825.2000.tb00328.x.

DOI:10.1111/j.1601-0825.2000.tb00328.x

PMID:10822360

Abstract

OBJECTIVES

Adherence of Candida albicans has been implicated as the first step in the pathogenesis of oral candidosis, and germ tube formation a contributory attribute. Recently, these organisms have also been implicated in persistent apical periodontitis. Chlorhexidine gluconate is by far the commonest antiseptic mouth wash prescribe in dentistry. As the intraoral concentrations of this antiseptic fluctuate considerably due to the dynamics of the oral cavity, the main objective of this study was to investigate the effect of brief exposure to three different sub-therapeutic concentrations of chlorhexidine gluconate (0.005%, 0.0025% and 0.00125%) on the germ tube formation of C. albicans. These findings were then correlated with the chlorhexidine-induced post-antifungal effect (PAFE) values we obtained in a study using the identical organisms and experimental conditions.

DESIGN

Ten oral isolates of C. albicans were exposed to three different concentrations of chlorhexidine gluconate for 30 min, the antiseptic removed, and the germ tube formation of these isolates quantified following subsequent incubation in a germ tube inducing medium. The PAFE was evaluated by turbidometric measurement of growth.

RESULTS

When compared with the controls, exposure to 0.005%, 0.0025% and 0.00125% chlorhexidine gluconate suppressed the ability to form germ tubes by 81.23% (P < 0.01), 42.74% (P < 0.01) and 9.13% (P > 0.05), respectively, while eliciting a mean PAFE of 9.91 h, 1.65 h and 0.67 h respectively. On regression analysis a significant positive correlation was observed between these two parameters (P < 0.0001; r = 0.7325).

CONCLUSIONS

Taken together, these findings imply that short exposure to sub-therapeutic levels of chlorhexidine gluconate may modulate candidal germ tube formation as well as its growth, thereby suppressing its pathogencity in vivo.

摘要

目的

白色念珠菌的黏附被认为是口腔念珠菌病发病机制的第一步，而芽管形成是一个促成因素。最近，这些微生物也与慢性根尖周炎有关。葡萄糖酸氯己定是牙科中最常用的抗菌漱口水。由于口腔环境的动态变化，这种抗菌剂在口腔内的浓度波动很大，本研究的主要目的是调查短暂暴露于三种不同亚治疗浓度的葡萄糖酸氯己定（0.005%、0.0025%和0.00125%）对白色念珠菌芽管形成的影响。然后将这些结果与我们在一项使用相同微生物和实验条件的研究中获得的氯己定诱导的抗真菌后效应（PAFE）值相关联。

设计

将10株白色念珠菌口腔分离株暴露于三种不同浓度的葡萄糖酸氯己定中30分钟，去除抗菌剂，然后在芽管诱导培养基中孵育后对这些分离株的芽管形成进行定量。通过比浊法测量生长来评估PAFE。

结果

与对照组相比，暴露于0.005%、0.0025%和0.00125%葡萄糖酸氯己定分别使芽管形成能力抑制了81.23%（P<0.01）、42.74%（P<0.01）和9.13%（P>0.05），同时分别产生平均PAFE为9.91小时、1.65小时和0.67小时。回归分析显示这两个参数之间存在显著正相关（P<0.0001；r=0.7325）。