Dumont F
Ann Immunol (Paris). 1975 Jun-Jul;126C(4):453-9.
Nude spleen (B) cells were cultivated in vitro in a serum-free medium, without or with lipopolysaccharide (LPS) and their surface-charge was measured by cell electrophoresis. In the absence of LPS, surface-charge did not vary significantly with time, whereas, in the presence of LPS a biphasic modification was observed. Thus, after 4 hours of culture with LPS, surface-charge was diminished by 14%. Subsequently, when DNA synthesis and blast formation were activated, it increased by up to 19-23% over control value, whether LPS was present continuously in the medium or had been eliminated by washing after 4 hours of incubation.
裸脾（B）细胞在无血清培养基中进行体外培养，培养时添加或不添加脂多糖（LPS），并通过细胞电泳测量其表面电荷。在没有LPS的情况下，表面电荷不会随时间显著变化，而在有LPS的情况下，则观察到双相修饰。因此，在用LPS培养4小时后，表面电荷减少了14%。随后，当DNA合成和母细胞形成被激活时，无论培养基中是否持续存在LPS，或者在培养4小时后通过洗涤将其去除，表面电荷都会比对照值增加高达19% - 23%。
