Yang C, Dash B C, Simon F, van der Groen G, Pieniazek D, Gao F, Hahn B H, Lal R B
HIV Immunology and Diagnostics Branch, Division of AIDS, STD, and TB Laboratory Research, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
J Infect Dis. 2000 May;181(5):1791-5. doi: 10.1086/315439. Epub 2000 May 15.
Human immunodeficiency virus type 1 (HIV-1) infection of humans is the result of independent cross-species transmissions of simian immunodeficiency viruses (SIVcpz) from naturally infected chimpanzees (Pan troglodytes troglodytes) to man. To develop a polymerase chain reaction-based assay capable of detecting members of all major phylogenetic SIVcpz and HIV-1 lineages (groups M, N, and O), primer pairs in conserved pol and env regions were designed. Both primer sets amplified </=10 copies of selected group M reference clones (subtypes A-H), proviral DNA or RNA of group N (YBF30), and group O of HIV-1 and also amplified divergent SIVcpz from cultured isolates (SIVcpzGAB1 and SIVcpzANT), uncultured spleen tissue (SIVcpzUS), and plasma (SIVcpzANT and SIVcpzUS). Sequences of the 2 amplicons (445 bp for gp41 and 261 bp for integrase) are of sufficient length for phylogenetic analyses, allowing both group and subtype classifications of the human viruses. Finally, both primer pairs are highly sensitive (>99%) in amplifying viral sequences from plasma taken from patients infected with HIV-1 group M (n=226) and O (n=17) viruses.
人类免疫缺陷病毒1型（HIV-1）感染人类是猿猴免疫缺陷病毒（SIVcpz）从自然感染的黑猩猩（Pan troglodytes troglodytes）独立跨物种传播给人类的结果。为了开发一种基于聚合酶链反应的检测方法，能够检测所有主要系统发育的SIVcpz和HIV-1谱系（M组、N组和O组）的成员，在保守的pol和env区域设计了引物对。两组引物均能扩增≤10个选定的M组参考克隆（A-H亚型）、N组（YBF30）的前病毒DNA或RNA以及HIV-1的O组，还能从培养分离株（SIVcpzGAB1和SIVcpzANT）、未培养的脾脏组织（SIVcpzUS）和血浆（SIVcpzANT和SIVcpzUS）中扩增出不同的SIVcpz。这两个扩增子（gp41为445 bp，整合酶为261 bp）的序列长度足以进行系统发育分析，从而对人类病毒进行组和亚型分类。最后，两组引物在扩增来自感染HIV-1 M组（n = 226）和O组（n = 17）病毒患者血浆中的病毒序列时具有高度敏感性（>99%）。
