Downing R, Pieniazek D, Hu D J, Biryahwaho B, Fridlund C, Rayfield M A, Sempala S D, Lal R B
Uganda Virus Research Institute, Entebbe.
AIDS Res Hum Retroviruses. 2000 May 20;16(8):815-9. doi: 10.1089/088922200308819.
To better understand the emergence of subtype C and its potential impact on vaccine efforts in Uganda, we have characterized subtype C sequences from Uganda (n = 13), Zimbabwe (n = 11), Mozambique (n = 5), South Africa (n = 4), and India (n = 3). Phylogenetic analysis of subtype C sequences in the env gp41 gene region revealed multiple subclusters within subtype C. Further, while most Ugandan specimen subclustered together, other subclusters did not reflect a clear geographic location. The nucleotide divergence within the Ugandan subset was 8.2% (6.1-9.8%) compared with 9.5% (2.5-15%) for the other subtype C gp41 sequences. The protein sequence alignment revealed marked sequence conservation of major immunodominant epitopes within the gp41 region.
为了更好地了解C亚型的出现及其对乌干达疫苗研发工作的潜在影响，我们对来自乌干达（n = 13）、津巴布韦（n = 11）、莫桑比克（n = 5）、南非（n = 4）和印度（n = 3）的C亚型序列进行了特征分析。对env gp41基因区域的C亚型序列进行系统发育分析，结果显示C亚型内存在多个亚簇。此外，虽然大多数乌干达样本聚集在一起，但其他亚簇并未反映出明确的地理位置。乌干达样本子集内的核苷酸差异为8.2%（6.1 - 9.8%），而其他C亚型gp41序列的核苷酸差异为9.
