Agnihotri Kalpana Dhiraj, Tripathy Srikanth P, Jere Abhay P, Kale Sameer M, Paranjape Ramesh S
Department of Molecular Virology, National AIDS Research Institute, G-73 MIDC, Bhosari, Pune, India.
J Acquir Immune Defic Syndr. 2006 Mar;41(3):345-51. doi: 10.1097/01.qai.0000209898.67007.1a.
Sequence polymorphism in HIV type 1 env gene is quite high, and there are little data available for subtype C env gp41 sequences from India. We have presented a molecular sequence analysis for gp41 region of env gene from HIV type 1 subtype C-infected individuals. The samples were obtained from 3 acute seroconverters and 5 seropositive individuals from India, one of whom was a minor. Heteroduplex mobility analysis using V3V5 and gp41 confirmed subtype C infection in all the study subjects. The sequences were analyzed for heterogeneity, polymorphism, and epitope recognition. The phylogenetic and SimPlot analysis showed the monophyletic lineage of Indian sequences. The phylogenetic tree constructed for the 286- to 506-bp region is highly variable and clearly distinguishes the subtype C sequences. The interpatient sequence comparison revealed high genetic diversity ranging from 0.0623 to 2.18 (median, 0.119). This supports the phylogeny where sequences belonging to the 8 study subjects form subclusters within Indian subtype C. A majority of the functional domains of gp41 are well conserved for the seroconverter and seropositive sequences. However, sequence polymorphism is high for the sequences obtained from the minor. The sequences of gp41 would provide valuable information regarding the diversity and its diagnostic implications in HIV/AIDS research.
1型人类免疫缺陷病毒（HIV-1）env基因的序列多态性相当高，而关于印度C亚型env gp41序列的可用数据很少。我们对来自1型HIV-1 C亚型感染个体的env基因gp41区域进行了分子序列分析。样本取自3名急性血清转化者和5名来自印度的血清阳性个体，其中1名是未成年人。使用V3V5和gp41进行异源双链迁移率分析证实所有研究对象均为C亚型感染。对序列进行了异质性、多态性和表位识别分析。系统发育和SimPlot分析显示了印度序列的单系谱系。为286至506碱基对区域构建的系统发育树高度可变，能清楚区分C亚型序列。患者间序列比较显示遗传多样性较高，范围为0.0623至2.18（中位数为0.119）。这支持了系统发育关系，即属于8名研究对象的序列在印度C亚型内形成亚簇。对于血清转化者和血清阳性序列，gp41的大多数功能域保存良好。然而，从未成年人获得的序列多态性较高。gp41序列将为HIV/AIDS研究中的多样性及其诊断意义提供有价值的信息。
