Shulman J M, Benton R, St Johnston D
Wellcome/CRC Institute and Department of Genetics, University of Cambridge, United Kingdom.
Cell. 2000 May 12;101(4):377-88. doi: 10.1016/s0092-8674(00)80848-x.
In C. elegans, the PAR-1 kinase is localized to the posterior of the zygote and is required for anterior-posterior axis formation. Here, we report that a Drosophila PAR-1 homolog localizes to the posterior of the oocyte with oskar mRNA. Furthermore, par-1 mutants show a novel polarity phenotype in which bicoid mRNA accumulates normally at the anterior, but oskar mRNA is redirected to the center of the oocyte, resulting in embryonic patterning defects. These phenotypes arise from a disorganization of the oocyte microtubule cytoskeleton, consistent with reports that mammalian PAR-1 homologs regulate microtubule dynamics. Thus, Drosophila PAR-1 may remodel the oocyte microtubule network to define the posterior as the site for oskar localization. These results identify a molecular parallel between anterior-posterior polarization in Drosophila and C. elegans.
在秀丽隐杆线虫中，PAR-1激酶定位于受精卵的后部，是前后轴形成所必需的。在此，我们报告果蝇PAR-1同源物与osk基因mRNA一起定位于卵母细胞的后部。此外，par-1突变体表现出一种新的极性表型，其中bicoid基因mRNA正常地在前部积累，但osk基因mRNA被重新引导至卵母细胞的中心，导致胚胎模式形成缺陷。这些表型源于卵母细胞微管细胞骨架的紊乱，这与哺乳动物PAR-1同源物调节微管动力学的报道一致。因此，果蝇PAR-1可能重塑卵母细胞微管网络，将后部定义为osk基因定位的位点。这些结果确定了果蝇和秀丽隐杆线虫前后极化之间的分子相似性。
