基于70千道尔顿热休克蛋白（HSP70）基因的隐孢子虫寄生虫的系统发育关系。
Phylogenetic relationships of Cryptosporidium parasites based on the 70-kilodalton heat shock protein (HSP70) gene.
作者信息
Sulaiman I M, Morgan U M, Thompson R C, Lal A A, Xiao L
机构信息
Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Services, U.S. Department of Health and Human Services, Atlanta, Georgia 30341, USA.
出版信息
Appl Environ Microbiol. 2000 Jun;66(6):2385-91. doi: 10.1128/AEM.66.6.2385-2391.2000.
We have characterized the nucleotide sequences of the 70-kDa heat shock protein (HSP70) genes of Cryptosporidium baileyi, C. felis, C. meleagridis, C. muris, C. serpentis, C. wrairi, and C. parvum from various animals. Results of the phylogenetic analysis revealed the presence of several genetically distinct species in the genus Cryptosporidium and eight distinct genotypes within the species C. parvum. Some of the latter may represent cryptic species. The phylogenetic tree constructed from these sequences is in agreement with our previous results based on the small-subunit rRNA genes of Cryptosporidium parasites. The Cryptosporidium species formed two major clades: isolates of C. muris and C. serpentis formed the first major group, while isolates of C. felis, C. meleagridis, C. wrairi, and eight genotypes of C. parvum formed the second major group. Sequence variations were also observed between C. muris isolates from ruminants and rodents. The HSP70 gene provides another useful locus for phylogenetic analysis of the genus Cryptosporidium.
我们已经对来自各种动物的贝利隐孢子虫、猫隐孢子虫、火鸡隐孢子虫、小鼠隐孢子虫、蛇隐孢子虫、怀氏隐孢子虫和微小隐孢子虫的70 kDa热休克蛋白（HSP70）基因的核苷酸序列进行了特征分析。系统发育分析结果显示，隐孢子虫属中存在几个遗传上不同的物种，微小隐孢子虫物种内有八个不同的基因型。其中一些可能代表隐性物种。根据这些序列构建的系统发育树与我们之前基于隐孢子虫寄生虫小亚基rRNA基因的结果一致。隐孢子虫物种形成了两个主要分支：小鼠隐孢子虫和蛇隐孢子虫的分离株形成了第一个主要群体，而猫隐孢子虫、火鸡隐孢子虫、怀氏隐孢子虫的分离株以及微小隐孢子虫的八个基因型形成了第二个主要群体。在反刍动物和啮齿动物的小鼠隐孢子虫分离株之间也观察到了序列变异。HSP70基因提供了另一个用于隐孢子虫属系统发育分析的有用位点。
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