Lee J, Kim D, Hong H, Han S, Kim J
Department of Anatomy, College of Veterinary Medicine, Chonnam National University, Kwangju, South Korea.
Neurosci Lett. 2000 Jun 9;286(3):179-82. doi: 10.1016/s0304-3940(00)01118-6.
The goal of this study was to determine whether etomidate has protective effect against kainic acid (KA)-induced neurotoxicity. Administration of etomidate (20 mg/kg i.p.) was performed in sequence, first being just 1 h after KA (10 mg/kg i.p.) injection, then three times at 1-h intervals. Neuronal damages in hippocampus were evaluated by using the acid fuchsin stain to detect cell death and the heat shock protein-70 (HSP-70) induction as an index of cell injury at 24 h after the administration of KA. HSP-70 induction and acid fuchsin positive neurons were increased in CA1 and CA3 regions of hippocampus after KA injection but significantly decreased by etomidate-injection. These results suggest that the etomidate hold potential effect on the protection of neurons against KA-induced neurotoxicity.
本研究的目的是确定依托咪酯是否对 kainic 酸（KA）诱导的神经毒性具有保护作用。依次给予依托咪酯（20mg/kg腹腔注射），首次在KA（10mg/kg腹腔注射）注射后1小时，然后每隔1小时给药3次。在给予KA后24小时，通过使用酸性品红染色检测细胞死亡以及诱导热休克蛋白70（HSP-70）作为细胞损伤指标来评估海马体中的神经元损伤。KA注射后，海马体CA1和CA3区域的HSP-70诱导和酸性品红阳性神经元增加，但依托咪酯注射后显著减少。这些结果表明，依托咪酯对保护神经元免受KA诱导的神经毒性具有潜在作用。
