通过荧光原位杂交技术在慢性淋巴细胞白血病/小淋巴细胞淋巴瘤的存档细胞病理学材料上检测12号染色体三体。
Detection of trisomy 12 by fluorescence in situ hybridization on archival cytopathologic material in chronic lymphocytic leukemia/small lymphocytic lymphoma.
作者信息
Liu K, Dodd L G, Powers J A, Bigner S H
机构信息
Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710, USA.
出版信息
Acta Cytol. 2000 May-Jun;44(3):368-74. doi: 10.1159/000328480.
OBJECTIVE
To evaluate fluorescence in situ hybridization (FISH) for the detection of trisomy 12 in archival cytologic specimens of chronic lymphocytic leukemia/small lymphocytic lymphoma.
STUDY DESIGN
The cytopathology database was searched for all cases of chronic lymphocytic leukemia/small lymphocytic lymphoma. Six cases of chronic lymphocytic leukemia/small lymphocytic lymphoma obtained by fine needle aspiration and one case of small lymphocytic lymphoma with plasmacytoid features were analyzed for trisomy 12 by FISH. These cases had been archived between 1 week to 16 months prior to analysis.
RESULTS
We detected trisomy 12 in four of the six cases of small lymphocytic lymphoma/chronic lymphocytic leukemia. The case of small lymphocytic lymphoma with plasmacytoid features was negative for trisomy 12.
CONCLUSION
Detection of trisomy 12 by FISH can be effectively performed on routinely prepared, stained and coverslipped archival cytologic material.
目的
评估荧光原位杂交（FISH）技术在慢性淋巴细胞白血病/小淋巴细胞淋巴瘤存档细胞学标本中检测12号染色体三体的应用。
研究设计
在细胞病理学数据库中搜索所有慢性淋巴细胞白血病/小淋巴细胞淋巴瘤病例。对6例通过细针穿刺获取的慢性淋巴细胞白血病/小淋巴细胞淋巴瘤病例以及1例具有浆细胞样特征的小淋巴细胞淋巴瘤病例，采用FISH技术分析12号染色体三体情况。这些病例在分析前已存档1周至16个月。
结果
在6例小淋巴细胞淋巴瘤/慢性淋巴细胞白血病病例中，我们检测到4例存在12号染色体三体。具有浆细胞样特征的小淋巴细胞淋巴瘤病例12号染色体三体检测结果为阴性。
结论
利用FISH技术检测12号染色体三体可在常规制备、染色并加盖盖玻片的存档细胞学材料上有效进行。
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