Yoshikawa Y, Mukai H, Hino F, Asada K, Kato I
Advanced Biomedical Center, Takara Shuzo Co., Ltd., 2257 Sunaike, Noji, Kusatsu, Shiga 525-0055, Japan.
Jpn J Cancer Res. 2000 May;91(5):459-63. doi: 10.1111/j.1349-7006.2000.tb00967.x.
Using a differential display technique, we identified two genes that are down-regulated in human gastric cancer tissue as compared to normal gastric mucosa. The down-regulated expression of these genes in gastric cancer tissue was confirmed by northern blotting analysis and RT-PCR. One, CA11, was a novel gene expressed predominantly in the stomach and was depleted in all of the gastric cancer cell lines examined. The other gene, GC36, was homologous to the digestive tract-specific calpain gene, nCL-4. The expression of both GC36 and nCL-4 was suppressed or depleted in gastric cancer cell lines of differentiated and poorly differentiated types. This is the first report of genes, the expression of which is down-regulated with considerable frequency in gastric cancer.
运用差异显示技术，我们鉴定出两个与正常胃黏膜相比在人胃癌组织中表达下调的基因。通过Northern印迹分析和逆转录聚合酶链反应（RT-PCR）证实了这些基因在胃癌组织中的表达下调。其中一个基因CA11是一个主要在胃中表达的新基因，在所检测的所有胃癌细胞系中均缺失。另一个基因GC36与消化道特异性钙蛋白酶基因nCL-4同源。在分化型和低分化型胃癌细胞系中，GC36和nCL-4的表达均受到抑制或缺失。这是首次报道在胃癌中表达频繁下调的基因。
