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A simple and rapid electrophoresis method to detect sequence variation in PCR-amplified DNA fragments.

作者信息

Wawer C, Rüggeberg H, Meyer G, Muyzer G

机构信息

Max-Planck-Institute for Marine Microbiology, Bremen, Germany.

出版信息

Nucleic Acids Res. 1995 Dec 11;23(23):4928-9. doi: 10.1093/nar/23.23.4928.

DOI:10.1093/nar/23.23.4928
PMID:8532541
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC307487/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c295/307487/a16c42adc90f/nar00023-0195-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c295/307487/2f941569fada/nar00023-0194-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c295/307487/a16c42adc90f/nar00023-0195-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c295/307487/2f941569fada/nar00023-0194-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c295/307487/a16c42adc90f/nar00023-0195-a.jpg

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SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.DNA大小与形状的沉降研究
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Genetic diversity of Desulfovibrio spp. in environmental samples analyzed by denaturing gradient gel electrophoresis of [NiFe] hydrogenase gene fragments.通过[NiFe]氢化酶基因片段的变性梯度凝胶电泳分析环境样品中脱硫弧菌属的遗传多样性。
Microglial activation varies in different models of Creutzfeldt-Jakob disease.
在不同的克雅氏病模型中，小胶质细胞的激活情况有所不同。
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Detection of a single base exchange in PCR-amplified DNA fragments using agarose gel electrophoresis containing bisbenzimide-PEG.使用含有双苯甲酰亚胺-聚乙二醇的琼脂糖凝胶电泳检测聚合酶链反应扩增的DNA片段中的单个碱基交换
Nucleic Acids Res. 1997 Dec 15;25(24):5125-6. doi: 10.1093/nar/25.24.5125.
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Distribution of bacterioplankton in meromictic Lake Saelenvannet, as determined by denaturing gradient gel electrophoresis of PCR-amplified gene fragments coding for 16S rRNA.通过对编码16S rRNA的基因片段进行PCR扩增后进行变性梯度凝胶电泳，确定了半混合湖Saelenvannet中浮游细菌的分布。
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DNA fingerprinting techniques for microorganisms. A proposal for classification and nomenclature.微生物的DNA指纹技术。分类与命名建议
Mol Biotechnol. 1996 Oct;6(2):115-42. doi: 10.1007/BF02740768.
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Polyethylene glycol derivatives of base and sequence specific DNA ligands: DNA interaction and application for base specific separation of DNA fragments by gel electrophoresis.碱基和序列特异性DNA配体的聚乙二醇衍生物：DNA相互作用以及通过凝胶电泳进行DNA片段碱基特异性分离的应用
Nucleic Acids Res. 1981 Jan 10;9(1):95-119. doi: 10.1093/nar/9.1.95.
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DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with melting theory.通过单碱基对替换而不同的DNA片段在变性梯度凝胶中被分离：与解链理论的对应关系。
Proc Natl Acad Sci U S A. 1983 Mar;80(6):1579-83. doi: 10.1073/pnas.80.6.1579.
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Cloning, characterization, and sequencing of the genes encoding the large and small subunits of the periplasmic [NiFe]hydrogenase of Desulfovibrio gigas.巨大脱硫弧菌周质[NiFe]氢化酶大亚基和小亚基编码基因的克隆、特性分析及测序
DNA. 1987 Dec;6(6):539-51. doi: 10.1089/dna.1987.6.539.
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Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms.通过凝胶电泳检测人类DNA多态性作为单链构象多态性。
Proc Natl Acad Sci U S A. 1989 Apr;86(8):2766-70. doi: 10.1073/pnas.86.8.2766.
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Temperature-gradient gel electrophoresis. Thermodynamic analysis of nucleic acids and proteins in purified form and in cellular extracts.温度梯度凝胶电泳。纯化形式及细胞提取物中核酸与蛋白质的热力学分析。
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Cloning and sequencing of a [NiFe] hydrogenase operon from Desulfovibrio vulgaris Miyazaki F.来自普通脱硫弧菌宫崎F株的[NiFe]氢化酶操纵子的克隆与测序
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