Balassa G, Milhaud P, Sousa J C, Silva M T
J Gen Microbiol. 1979 Feb;110(2):381-92. doi: 10.1099/00221287-110-2-381.
In decadent sporulation mutants, sporulating populations are heterogeneous: the cells reach successive chemical and physical resistances with progressively decreasing frequencies. Each decadent mutant can be characterized by the shape and slope of the curve describing the frequency of cells resistant to various agents ('the resistance spectrum'). In some mutants the resistance spectrum decreases progressively from xylene resistance to heat resistance; in other mutants it decreases rapidly between octanol resistance and chloroform resistance. Electron microscopy showed that in two mutants the majority of the cells are blocked at stages III and IV; the number of cells that develop further to reach successive morphological stages falls off progressively. In two other mutants most cells reach stage V. Cortexless spores are also frequent. One of the decadent mutations, SpoL1, was localized between aroD and acf. The phenotype of decadent mutants is discussed in terms of sequential gene activation.
在衰退型芽孢形成突变体中，进行芽孢形成的群体是异质的：细胞达到连续的化学和物理抗性，但其频率逐渐降低。每个衰退型突变体都可以通过描述对各种试剂具有抗性的细胞频率的曲线形状和斜率来表征（“抗性谱”）。在一些突变体中，抗性谱从对二甲苯抗性到耐热性逐渐降低；在其他突变体中，它在对辛醇抗性和对氯仿抗性之间迅速降低。电子显微镜显示，在两个突变体中，大多数细胞在III期和IV期受阻；进一步发育以达到连续形态阶段的细胞数量逐渐减少。在另外两个突变体中，大多数细胞达到V期。无皮层孢子也很常见。其中一个衰退型突变体SpoL1定位于aroD和acf之间。根据顺序基因激活来讨论衰退型突变体的表型。
