Errington J, Mandelstam J
J Gen Microbiol. 1984 Aug;130(8):2115-21. doi: 10.1099/00221287-130-8-2115.
Twenty-nine mutants blocked during stage V of sporulation have been isolated following directed mutagenesis of the lys-1 region of the Bacillus subtilis 168 chromosome. All of a sample of eight mutants tested are unaffected in sporulation marker events up to stage IV but did not produce dipicolinic acid. They produced stable 'phase white' spores that were released from the mother cell, and were partially resistant to toluene and lysozyme but sensitive to chloroform and heat. Mutation spoV A89, known to be in the lys-1 region, showed similar phenotypic characteristics. Three-factor transformation crosses and recombination indices showed that the new mutations and spoV A89 lie in a single linkage group, which maps between lys-1 and another sporulation locus, spoIIA. The size of the spoV A locus is such that it probably contains several genes, and these may be contiguous with the cluster of genes included within the spoIIA locus.
在对枯草芽孢杆菌168染色体的lys-1区域进行定向诱变后，已分离出29个在芽孢形成V期受阻的突变体。所测试的8个突变体样本中的所有突变体在IV期之前的芽孢形成标记事件中均未受影响，但未产生二吡啶羧酸。它们产生了稳定的“阶段白色”孢子，这些孢子从母细胞中释放出来，对甲苯和溶菌酶部分抗性，但对氯仿和热敏感。已知位于lys-1区域的突变体spoV A89表现出相似的表型特征。三因子转化杂交和重组指数表明，新的突变体和spoV A89位于一个单一的连锁群中，该连锁群定位在lys-1和另一个芽孢形成位点spoIIA之间。spoV A位点的大小表明它可能包含几个基因，并且这些基因可能与spoIIA位点内的基因簇相邻。
