Dus Santos M J, Wigdorovitz A, Maradei E, Periolo O, Smitsaart E, Borca M V, Sadir A M
Instituto de Virología, CICV, INTA, Buenos Aires, Argentina.
Vet Res Commun. 2000 May;24(4):261-73. doi: 10.1023/a:1006450900739.
We present a comparison of methods for evaluating the potency of foot and mouth disease vaccine in the laboratory. The anti-FMDV antibodies (Ab) in vaccinated mice were tested by liquid phase (lp) ELISA, solid phase (sp) ELISA and virus neutralization (VN), and were compared with the Ab titres detected by lpELISA, which is the official test in Argentina for testing the potency of FMD vaccines and protection against a virulent challenge in cattle. The results demonstrated that it is possible to relate the Ab levels induced in vaccinated mice with both the Ab and protective responses elicited in cattle. Furthermore, it was found that the anti-FMDV Ab titres in mice detected by lpELISA 14 days after vaccination should be an accurate parameter for predicting the results of the challenge test in cattle. Thus, this test in mice appears to be an inexpensive and rapid alternative for testing FMD vaccines in cattle.
我们展示了实验室中评估口蹄疫疫苗效力的方法比较。通过液相（lp）ELISA、固相（sp）ELISA和病毒中和（VN）检测接种疫苗小鼠体内的抗口蹄疫病毒抗体（Ab），并与通过lpELISA检测的抗体滴度进行比较，lpELISA是阿根廷检测口蹄疫疫苗效力和预防牛感染强毒攻击的官方检测方法。结果表明，接种疫苗小鼠体内诱导的抗体水平与牛体内引发的抗体和保护反应相关。此外，发现接种疫苗14天后通过lpELISA检测的小鼠抗口蹄疫病毒抗体滴度应是预测牛攻毒试验结果的准确参数。因此，小鼠中的这项检测似乎是一种用于检测牛口蹄疫疫苗的廉价且快速的替代方法。
