Borca M V, Fernández F M, Sadir A M, Schudel A A
Vet Microbiol. 1984 Dec;10(1):1-11. doi: 10.1016/0378-1135(84)90051-8.
Passive transfer experiments were performed to serve as a basis for analyzing the immune response of adult mice to FMDV infection. Animals were irradiated (750 rad: 1 lethal dose 50%) and reconstituted with allogeneic mononuclear cells from blood, spleen, thymus and peritoneal cavity from donors 2 and 8 days post-inoculation (p.i.). Donors were primed with 10 000 suckling mouse 50% lethal doses of FMDV strain O1 Campos. The following parameters were studied in recipient mice challenged with 10 000 suckling mouse 50% lethal doses of the same virus: (1) viremia; (b) FMDV neutralizing antibody titres; (c) sheep red blood cell (SRBC) hemagglutinating antibody titres. Viremia was substantially prolonged in irradiated control mice, which did not produce detectable antibodies to FMDV or SRBC. In contrast, the span of viremia was markedly shorter in animals reconstituted with cells obtained 8 days p.i. and its eclipse coincided with the onset of neutralizing antibody production. An equally efficient antibody response to the inoculation of SRBC was observed in these animals. No effect was detected after the transfer of cells obtained 2 days p.i. It is concluded that the humoral immune response plays a predominant role in the recovery from FMDV experimental infection in adult mice.
进行了被动转移实验，以此作为分析成年小鼠对口蹄疫病毒（FMDV）感染免疫反应的基础。对动物进行辐照（750拉德：1个半数致死剂量的50%），并在接种后第2天和第8天用来自供体血液、脾脏、胸腺和腹腔的同种异体单核细胞进行重建。供体用10000个乳鼠50%致死剂量的口蹄疫病毒O1坎波斯毒株进行免疫。在接受10000个乳鼠50%致死剂量相同病毒攻击的受体小鼠中研究了以下参数：（1）病毒血症；（b）口蹄疫病毒中和抗体滴度；（c）绵羊红细胞（SRBC）血凝抗体滴度。在未产生可检测到的口蹄疫病毒或绵羊红细胞抗体的辐照对照小鼠中，病毒血症显著延长。相比之下，用接种后第8天获得的细胞重建的动物，其病毒血症持续时间明显缩短，且其隐蔽期与中和抗体产生的开始时间一致。在这些动物中观察到对绵羊红细胞接种的抗体反应同样有效。接种后第2天获得的细胞转移后未检测到效果。得出的结论是，体液免疫反应在成年小鼠从口蹄疫病毒实验性感染中恢复过程中起主要作用。
