Okimotoa Y, Watanabea A, Nikia E, Yamashitab T, Noguchia N
Research Center for Advanced Science and Technology, University of Tokyo, Meguro, Japan.
FEBS Lett. 2000 Jun 2;474(2-3):137-40. doi: 10.1016/s0014-5793(00)01587-8.
Diphenyl-1-pyrenylphosphine (DPPP) was tested whether it could be used as a fluorescent probe to monitor lipid peroxidation in cell membranes. DPPP reacted with organic hydroperoxides and hydrogen peroxide stoichiometrically to give DPPP oxide (DPPP = O). DPPP incorporated into phosphatidylcholine liposomal membranes and polymorphonuclear leukocytes (PMNs) reacted with methyl linoleate hydroperoxide rapidly but not with hydrogen peroxide nor with tert-butyl hydroperoxide. This novel method revealed that lipid peroxidation proceeded within membranes of PMNs stimulated with phorbol 12-myristate 13-acetate, which is known to produce several kinds of free radicals. It was found that DPPP is a suitable fluorescent probe to monitor lipid peroxidation within cell membranes specifically.
测试了二苯基-1-芘基膦（DPPP）是否可作为荧光探针来监测细胞膜中的脂质过氧化。DPPP与有机氢过氧化物和过氧化氢按化学计量反应生成二苯基-1-芘基膦氧化物（DPPP = O）。掺入磷脂酰胆碱脂质体膜和多形核白细胞（PMN）中的DPPP与亚油酸甲酯氢过氧化物迅速反应，但不与过氧化氢和叔丁基氢过氧化物反应。这种新方法表明，在用佛波醇12-肉豆蔻酸酯13-乙酸酯刺激的PMN膜内发生了脂质过氧化，已知该酯会产生多种自由基。发现DPPP是一种特别适合监测细胞膜内脂质过氧化的荧光探针。
