Liu Xiao, Li Yunfei, Zhang Rong, Huangfu Lulu, Du Guihong, Xiang Qisen
College of Food and Biological Engineering, Zhengzhou University of Light Industry, No. 136, Kexue Road, Zhengzhou, 450001, People's Republic of China.
Henan Key Laboratory of Cold Chain Food Quality and Safety Control, Zhengzhou, 450001, People's Republic of China.
Appl Microbiol Biotechnol. 2021 Apr;105(7):2855-2865. doi: 10.1007/s00253-021-11227-9. Epub 2021 Mar 18.
The present study aimed to elucidate the antifungal effect and underlying mechanism of plasma-activated water (PAW) combined with sodium laureth sulfate (SLES) against Saccharomyces cerevisiae. S. cerevisiae, initially at 6.95 log colony-forming unit (CFU)/mL, decreased to an undetectable level following the synergistic treatment of PAW and SLES (0.50 mg/mL) for 20 min. After PAW treatment combined with SLES (2.5 mg/mL) for 30 min, the S. cerevisiae cells on polyethylene films also reduced to an undetectable level from the initial load of 5.84 log CFU/cm. PAW + SLES treatment caused severe disruption of membrane integrity and increased lipid oxidation within the cell membrane and the intracellular reactive oxygen species levels in S. cerevisiae cells. Besides, the disruption of the mitochondrial membrane potential (∆ψm) was also observed in S. cerevisiae cells after treatment of PAW and SLES at 0.01 mg/mL for 5 min. These data suggest that the combined treatment of PAW and SLES causes oxidation injury to cell membranes and abnormal ∆ψm in S. cerevisiae, which may be eventually responsible for cell death. This study demonstrates the potential application of PAW combined with SLES as an alternative disinfection method. Key Points • PAW + SLES exhibited synergistic antifungal activity against S. cerevisiae. • PAW + SLES resulted in severe disruption of membrane integrity and permeability. • PAW + SLES induced accumulation of reactive oxygen species in S. cerevisiae cells.
本研究旨在阐明等离子体活化水（PAW）与月桂醇聚醚硫酸酯钠（SLES）联合对酿酒酵母的抗真菌作用及其潜在机制。初始浓度为6.95 log菌落形成单位（CFU）/mL的酿酒酵母，经PAW与SLES（0.50 mg/mL）协同处理20分钟后，降至检测不到的水平。PAW与SLES（2.5 mg/mL）联合处理30分钟后，聚乙烯薄膜上的酿酒酵母细胞也从初始负载量5.84 log CFU/cm²降至检测不到的水平。PAW + SLES处理导致酿酒酵母细胞膜完整性严重破坏，细胞膜内脂质氧化增加，细胞内活性氧水平升高。此外，在0.01 mg/mL的PAW和SLES处理5分钟后，酿酒酵母细胞中也观察到线粒体膜电位（∆ψm）的破坏。这些数据表明，PAW和SLES联合处理对酿酒酵母细胞膜造成氧化损伤并导致∆ψm异常，这可能最终导致细胞死亡。本研究证明了PAW与SLES联合作为一种替代消毒方法的潜在应用。要点 • PAW + SLES对酿酒酵母表现出协同抗真菌活性。 • PAW + SLES导致膜完整性和通透性严重破坏。 • PAW + SLES诱导酿酒酵母细胞中活性氧的积累。
