Ozawa R, Arimura G, Takabayashi J, Shimoda T, Nishioka T
Bio-oriented Technology Research Advancement Institution, Tokyo, Japan.
Plant Cell Physiol. 2000 Apr;41(4):391-8. doi: 10.1093/pcp/41.4.391.
We compared volatiles from lima bean leaves (Phaseolus lunatus) infested by either beet armyworm (Spodoptera exigua), common armyworm [Mythimna (Pseudaletia) separata], or two-spotted spider mite (Tetranychus urticae). We also analyzed volatiles from the leaves treated with jasmonic acid (JA) and/or methyl salicylate (MeSA). The volatiles induced by aqueous JA treatment were qualitatively and quantitatively similar to those induced by S. exigua or M. separata damage. Furthermore, both S. exigua and aqueous JA treatment induced the expression of the same basic PR genes. In contrast, gaseous MeSA treatment, and aqueous JA treatment followed by gaseous MeSA treatment, induced volatiles that was qualitatively and quantitatively more similar to the T. urticae-induced volatiles than those induced by aqueous JA treatment. In addition, T. urticae damage resulted in the expression of the acidic and basic PR genes that were induced by gaseous MeSA treatment and by aqueous JA treatment, respectively. Based on these data, we suggest that in lima bean leaves, the JA-related signaling pathway is involved in the production of caterpillar-induced volatiles, while both the SA-related signaling pathway and the JA-related signaling pathway are involved in the production of T. urticae-induced volatiles.
我们比较了受甜菜夜蛾（Spodoptera exigua）、粘虫[Mythimna (Pseudaletia) separata]或二斑叶螨（Tetranychus urticae）侵害的利马豆叶片（Phaseolus lunatus）释放的挥发性物质。我们还分析了用茉莉酸（JA）和/或水杨酸甲酯（MeSA）处理过的叶片释放的挥发性物质。经JA水溶液处理诱导产生的挥发性物质在定性和定量上与受甜菜夜蛾或粘虫侵害诱导产生的挥发性物质相似。此外，甜菜夜蛾和JA水溶液处理均诱导了相同的基础病程相关基因的表达。相比之下，气态MeSA处理以及JA水溶液处理后再进行气态MeSA处理所诱导产生的挥发性物质，在定性和定量上比JA水溶液处理诱导产生的挥发性物质更类似于二斑叶螨诱导产生的挥发性物质。此外，二斑叶螨侵害分别导致了由气态MeSA处理和JA水溶液处理诱导的酸性和基础病程相关基因的表达。基于这些数据，我们认为在利马豆叶片中，JA相关信号通路参与了毛虫诱导的挥发性物质的产生，而SA相关信号通路和JA相关信号通路均参与了二斑叶螨诱导的挥发性物质的产生。
