Mohammad S F, Hardison M D, Chuang H Y, Mason R G
Haemostasis. 1976;5(2):96-114. doi: 10.1159/000214123.
Adhesiveness of washed platelets resuspended in citrated plasma, serum, or several different media has been investigated. A method specific for quanititation of adhesion was used. Platelets suspended in saline of Tyrode's solution were found to be highly adhesive to glass, polyethylene, polyvinyl chloride, or Cuprophane. This adhesiveness of platelets to test surfaces decreased by nearly 50% when plasma was the suspension medium. When the suspension medium was serum, the decrease in adhesion was nearly 75%. Cohn fraction V also decreased the adhesiveness of platelets significantly, but highly purfied albumin had only a small effect. Several pharmacologic agents decreased platelet adhesiveness when added to platelets suspended in plasma or serum, but had negligible effect on the adhesiveness of platelets suspended in artificial media devoid of proteins. Normal washed platelets, when suspended in citrated plasma obtained from an afibrinogenemic donor or in normal serum, showed a significant decrease in adhesion compared to the same platelets suspended in normal citrated plasma. Addition of fibrinogen to afibrinogenemic plasma or normal serum restored the adhesiveness of platelets to normal levels. Normal platelets resuspended in plasma obtained from a thrombasthenic donor exhibited normal adhesiveness. These observations suggested that while fibrinogen promotes platelet adhesion, plasma or serum possess also an adhesion-inhibiting activity.
已对重悬于枸橼酸盐血浆、血清或几种不同介质中的洗涤血小板的黏附性进行了研究。采用了一种特异性定量黏附性的方法。发现悬浮于生理盐水或台氏液中的血小板对玻璃、聚乙烯、聚氯乙烯或铜氨丝具有高度黏附性。当血浆作为悬浮介质时，血小板对测试表面的这种黏附性降低了近50%。当悬浮介质是血清时，黏附性降低了近75%。Cohn Ⅴ组分也显著降低了血小板的黏附性，但高度纯化的白蛋白只有很小的影响。几种药理剂添加到悬浮于血浆或血清中的血小板时会降低血小板黏附性，但对悬浮于不含蛋白质的人工介质中的血小板黏附性影响可忽略不计。正常洗涤血小板悬浮于来自无纤维蛋白原血症供体的枸橼酸盐血浆或正常血清中时，与悬浮于正常枸橼酸盐血浆中的相同血小板相比，黏附性显著降低。向无纤维蛋白原血症血浆或正常血清中添加纤维蛋白原可使血小板黏附性恢复到正常水平。重悬于来自血小板无力症供体的血浆中的正常血小板表现出正常的黏附性。这些观察结果表明，虽然纤维蛋白原促进血小板黏附，但血浆或血清也具有黏附抑制活性。
