核糖体DNA复制叉屏障与HOT1重组热点：共享序列但活动独立
Ribosomal DNA replication fork barrier and HOT1 recombination hot spot: shared sequences but independent activities.
作者信息
Ward T R, Hoang M L, Prusty R, Lau C K, Keil R L, Fangman W L, Brewer B J
机构信息
Department of Genetics, University of Washington, Seattle 98195, USA.
出版信息
Mol Cell Biol. 2000 Jul;20(13):4948-57. doi: 10.1128/MCB.20.13.4948-4957.2000.
Abstract
In the ribosomal DNA of Saccharomyces cerevisiae, sequences in the nontranscribed spacer 3' of the 35S ribosomal RNA gene are important to the polar arrest of replication forks at a site called the replication fork barrier (RFB) and also to the cis-acting, mitotic hyperrecombination site called HOT1. We have found that the RFB and HOT1 activity share some but not all of their essential sequences. Many of the mutations that reduce HOT1 recombination also decrease or eliminate fork arrest at one of two closely spaced RFB sites, RFB1 and RFB2. A simple model for the juxtaposition of RFB and HOT1 sequences is that the breakage of strands in replication forks arrested at RFB stimulates recombination. Contrary to this model, we show here that HOT1-stimulated recombination does not require the arrest of forks at the RFB. Therefore, while HOT1 activity is independent of replication fork arrest, HOT1 and RFB require some common sequences, suggesting the existence of a common trans-acting factor(s).
摘要
在酿酒酵母的核糖体DNA中，35S核糖体RNA基因非转录间隔区3'端的序列对于复制叉在一个称为复制叉屏障（RFB）的位点处的极性停滞以及对称为HOT1的顺式作用有丝分裂超重组位点都很重要。我们发现，RFB和HOT1活性共享一些但并非全部的必需序列。许多降低HOT1重组的突变也会减少或消除在两个紧密相邻的RFB位点之一（RFB1和RFB2）处的复制叉停滞。关于RFB和HOT1序列并列的一个简单模型是，在RFB处停滞的复制叉中的链断裂会刺激重组。与该模型相反，我们在此表明，HOT1刺激的重组并不需要复制叉在RFB处停滞。因此，虽然HOT1活性独立于复制叉停滞，但HOT1和RFB需要一些共同序列，这表明存在一种共同的反式作用因子。
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