López-estraño C, Schvartzman J B, Krimer D B, Hernández P
Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Velázquez 144, Madrid, 28006, Spain.
J Mol Biol. 1998 Mar 27;277(2):249-56. doi: 10.1006/jmbi.1997.1607.
We investigated the replication of the region where transcription terminates in mouse rDNA. It contains a replication fork barrier (RFB) that behaves in a polar manner, arresting only replication forks moving in the direction opposite to transcription. This RFB consists of several closely spaced fork arrest sites that co-localize with the transcription terminator elements, known as Sal boxes. Sal boxes are the target for mTTF-I (murine transcription termination factor I). These results suggest that both termination of rRNA transcription and replication fork arrest may share cis-acting as well as trans-acting factors.
我们研究了小鼠核糖体DNA中转录终止区域的复制情况。该区域包含一个复制叉障碍（RFB），其作用具有极性，仅阻止沿与转录相反方向移动的复制叉。这个RFB由几个紧密间隔的叉停滞位点组成，这些位点与转录终止元件（称为Sal盒）共定位。Sal盒是小鼠转录终止因子I（mTTF-I）的作用靶点。这些结果表明，rRNA转录的终止和复制叉停滞可能共享顺式作用和反式作用因子。
