Hurley C K, Maiers M, Ng J, Wagage D, Hegland J, Baisch J, Endres R, Fernandez-Vina M, Heine U, Hsu S, Kamoun M, Mitsuishi Y, Monos D, Noreen H, Perlee L, Rodriguez-Marino S, Smith A, Stastny P, Trucco M, Yang S Y, Yu N, Holsten R, Hartzman R J, Setterholm M
Department of Microbiology and Immunology, Georgetown University Medical Center, Washington, DC 20007, USA.
Tissue Antigens. 2000 Apr;55(4):352-8. doi: 10.1034/j.1399-0039.2000.550409.x.
DNA-based typing of HLA class I alleles of the HLA-A and HLA-B loci using sequence-specific oligonucleotide primers and/or probes has been used for the large-scale typing of individuals for the National Marrow Donor Program unrelated donor registry. Typing was performed by 16 laboratories at a low level of resolution (e.g. A01, B07). The results of blinded quality control analysis for the first 12 months of the project show the typing to be highly accurate, specific and reliable. The total error rate based on 11,545 HLA-A and 11,428 HLA-B assignments was 1.1% for HLA-A and 1.9% for HLA-B. This level of accuracy is particularly remarkable because the quality control samples could not be distinguished from 64,180 donor samples tested at the same time by the laboratories.
使用序列特异性寡核苷酸引物和/或探针，对HLA - A和HLA - B位点的HLA - I类等位基因进行基于DNA的分型，已用于国家骨髓捐献计划无关供者登记处的大规模个体分型。分型由16个实验室以低分辨率水平（例如A01，B07）进行。该项目前12个月的盲法质量控制分析结果表明，分型具有高度准确性、特异性和可靠性。基于11,545个HLA - A和11,428个HLA - B分型结果，HLA - A的总错误率为1.1%，HLA - B为1.9%。这一准确性水平尤为显著，因为质量控制样本与实验室同时检测的64,180个供者样本无法区分。
