Boas S R, Danduran M J, McColley S A, Beaman K, O'Gorman M R
Children's Memorial Hospital, Division of Pulmonary and Intensive Care Medicine, Chicago, Illinois 60614, USA.
Int J Sports Med. 2000 May;21(4):294-301. doi: 10.1055/s-2000-311.
Previous studies have demonstrated altered immune response following exercise in healthy adults and children. As data are lacking in children with cystic fibrosis, we evaluated the immune response following acute exercise and hypothesized that acute increases in cellular changes would be seen but would be blunted in subjects with CF. Leukocytes, lymphocytes, and their subsets as well as natural killer cell number and activity were determined before, immediately after, and one hour post exhaustive exercise in 15 children with cystic fibrosis (8-21 yrs, FEV1 69.5+/-18.0%, colonized with P aeruginosa) and 15 healthy controls (8-18 yrs, FEV1 107.5+/-10.7%). At baseline the cystic fibrosis group had greater leukocytes (9.25+/-2.83 vs. 5.17+/-0.96 x 10(9) cells/liter). Immediately post exercise, the cystic fibrosis group demonstrated increases in cell counts for leukocytes (32.4%), lymphocytes (61.8%), granulocytes (36.4%), monocytes (76.2%), and natural killer cells (315%). Similar percentage increases were seen in cell counts for the controls (leukocytes: 39.5%, lymphocytes: 78.5%, granulocytes: 32.0%, monocytes: 75.9%, and NK cells: 442%). Natural killer cell activity also increased by 57.9% in the group with cystic fibrosis and by 43.6% in the healthy controls. Except for elevated leukocyte and granulocyte counts, values returned to baseline at one hour post-exercise. In conclusion, the cellular immune response to acute exercise in children with mild to moderate cystic fibrosis appears normal.
先前的研究表明，健康成年人和儿童在运动后免疫反应会发生改变。由于囊性纤维化患儿的数据尚缺，我们评估了急性运动后的免疫反应，并假设在细胞变化方面会出现急性增加，但在囊性纤维化患者中这种增加会减弱。在15名囊性纤维化患儿（8 - 21岁，第一秒用力呼气量[FEV1]为69.5±18.0%，感染铜绿假单胞菌）和15名健康对照者（8 - 18岁，FEV1为107.5±10.7%）进行力竭运动前、运动后即刻以及运动后1小时，测定白细胞、淋巴细胞及其亚群以及自然杀伤细胞的数量和活性。在基线时，囊性纤维化组的白细胞数量更多（9.25±2.83对5.17±0.96×10⁹个细胞/升）。运动后即刻，囊性纤维化组的白细胞（32.4%）、淋巴细胞（61.8%）、粒细胞（36.4%）、单核细胞（76.2%）和自然杀伤细胞（315%）计数增加。对照组的细胞计数也出现了类似的百分比增加（白细胞：39.5%，淋巴细胞：78.5%，粒细胞：32.0%，单核细胞：75.9%，自然杀伤细胞：442%）。囊性纤维化组的自然杀伤细胞活性也增加了57.9%，健康对照组增加了43.6%。除白细胞和粒细胞计数升高外，运动后1小时各项数值恢复至基线水平。总之，轻度至中度囊性纤维化患儿对急性运动的细胞免疫反应似乎正常。
