人类和小鼠的周期蛋白1基因：五个高度保守的E盒对mPer1转录增强有累加作用。
The human and mouse Period1 genes: five well-conserved E-boxes additively contribute to the enhancement of mPer1 transcription.
作者信息
Hida A, Koike N, Hirose M, Hattori M, Sakaki Y, Tei H
机构信息
Laboratory of Structural Genomics, Human Genome Center, Institute of Medical Science, University of Tokyo, Japan.
出版信息
Genomics. 2000 May 1;65(3):224-33. doi: 10.1006/geno.2000.6166.
The clock gene, Period1, from human and mouse was sequenced and characterized. Both human PERIOD1 (human PER1) and mouse Period1 (mouse Per1) consisted of 23 exons spanning approximately 16 kb, and their structures showed strong similarity to each other. For example, six highly conserved regions were identified in the 5' upstream sequences. These conserved segments exhibited 77-88% identity and possessed several potential regulatory elements including five E-boxes (the binding site of the CLOCK-BMAL1 complex) and four cyclic AMP response elements. Transient transfection assays using a mPer1-luciferase fusion gene revealed that each of the conserved E-boxes additively functions as an enhancer for the transactivation of mPer1 by mCLOCK and mBMAL1.
对来自人类和小鼠的生物钟基因Period1进行了测序和特征分析。人类PERIOD1（hPER1）和小鼠Period1（mPer1）均由23个外显子组成，跨越约16 kb，它们的结构彼此显示出高度相似性。例如，在5'上游序列中鉴定出六个高度保守的区域。这些保守片段的同一性为77-88%，并具有几个潜在的调控元件，包括五个E-box（CLOCK-BMAL1复合物的结合位点）和四个环磷酸腺苷反应元件。使用mPer1-荧光素酶融合基因的瞬时转染试验表明，每个保守的E-box均可作为mCLOCK和mBMAL1对mPer1反式激活的增强子发挥累加作用。
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