Takács J, Markova L, Borostyánköi Z, Görcs T J, Hámori J
Neurobiology Research Group of the Hungarian Academy of Sciences and Semmelweis Medical University, Budapest.
J Neurosci Res. 1999 Mar 15;55(6):733-48. doi: 10.1002/(SICI)1097-4547(19990315)55:6<733::AID-JNR8>3.0.CO;2-8.
Morphology, distribution and number of unipolar brush cells (UBCs) was studied in the cerebellar vermal lobules I-X of the chicken, rat, guinea pig, cat, and monkey using monoclonal mGluR1a antibody as a marker to visualise these recently described nerve cells (Mugnaini and Floris [1994] J. Comp. Neurol. 339:174-180; Mugnaini et al. [1994] Synapse 16:284-311). The morphological appearance of mGluR1a immunopositive UBCs is similar in all species investigated: they are small cells, having a single, relatively short and thick dendrite, terminating in brush-like dendrioles. Although this, probably excitatory, cell type can be found all over the cerebellar cortex, highest density of UBCs can be seen in the vermal cortex. The present study, therefore, was focused on the quantitative morphology and distribution of UBCs in the 10 lobules of the vermis. Calculating the number of UBCs/l Purkinje cell (PC), we have found differences in this value (average in vermal lobules I-X) from 1.04 in rat, 1.10 in chicken, 1.16 in guinea pig, 2.27 in monkey, and up to 2.44 in cat. The highest density of UBCs was observed in lobules I, IX, and X, whereas the lowest number of UBCs/l PC was found in lobules IV-VI (in the mammals) and in lobules VII-VIII (in the chicken). In mammals, particularly the monkey and cat, an increased presence of UBCs was observed in vermal sub-lobules VIc-VIIb,c, a region defined as the oculomotor vermis because of its role in the control of saccadic eye movement. There is also a basic difference between chicken and mammals in the distribution of UBCs within the lobules: in mammals, the lowest density of these nerve cells was found in the peripheral portion of the lobules, near to the pia, while in the chicken, in contrast, the density of UBCs was the highest subpially with fewer UBCs located in the deepest curvature of the lobules. Finally, the functional significance of the differences in the density and in the distribution pattern of UBCs in the cerebellar vermis between the phylogenetically different species investigated is briefly discussed.
利用单克隆mGluR1a抗体作为标记物，来观察鸡、大鼠、豚鼠、猫和猴的小脑蚓部I-X小叶中，单极刷状细胞（UBCs）的形态、分布和数量，这些神经细胞是最近才被描述的（Mugnaini和Floris [1994] 《比较神经学杂志》339:174 - 180；Mugnaini等人 [1994] 《突触》16:284 - 311）。在所有被研究的物种中，mGluR1a免疫阳性的UBCs的形态外观相似：它们是小细胞，有一个单一的、相对短而粗的树突，末端为刷状小分支。虽然这种可能具有兴奋性的细胞类型在整个小脑皮质都能找到，但UBCs的最高密度出现在蚓部皮质。因此，本研究聚焦于蚓部10个小叶中UBCs的定量形态和分布。通过计算每浦肯野细胞（PC）中UBCs的数量，我们发现这个值（蚓部小叶I-X的平均值）在不同物种间存在差异：大鼠为1.04，鸡为1.10，豚鼠为1.16，猴为2.27，猫高达2.44。UBCs的最高密度出现在小叶I、IX和X，而每浦肯野细胞中UBCs数量最少的情况出现在小叶IV-VI（在哺乳动物中）和小叶VII-VIII（在鸡中）。在哺乳动物中，尤其是猴和猫，在蚓部亚小叶VIc-VIIb,c中观察到UBCs数量增加，该区域因在控制眼球扫视运动中的作用而被定义为动眼蚓部。鸡和哺乳动物在小叶内UBCs的分布上也存在根本差异：在哺乳动物中，这些神经细胞的最低密度出现在小叶靠近软膜的周边部分，而相比之下，在鸡中，UBCs的密度在软膜下最高，位于小叶最深弯曲处的UBCs较少。最后，简要讨论了在系统发育上不同的被研究物种之间，小脑蚓部UBCs密度和分布模式差异的功能意义。
