Tsubouchi H, Terada H, Yamamoto K, Hisada K, Sakabe Y
Mycopathologia. 1985 Jun;90(3):181-6. doi: 10.1007/BF00436735.
The growth and ochratoxin A production of Aspergillus ochraceus strains S-235-100 and IFM 0458, which were isolated from green coffee beans and glutinous rice, respectively, were examined in yeast extract-sucrose (YES) medium containing 0.1 to 1.0% caffeine. The mycelial growth and ochratoxin A formation of strain IFM 0458 was inhibited by caffeine at concentrations over 0.1%, and ochratoxin A was not produced at caffeine levels of 0.5% and 1.0%. Contrary to this, A. ochraceus strain S-235-100 produced a larger amount of ochratoxin A in the presence of 0.5% and 1.0% caffeine when grown on YES medium, reaching a maximum after 15 to 20 days of incubation. The formation of ochratoxin A by nine additional strains of A. ochraceus, three strains of A. elegans and one strain of A. sclerotiorum isolated from green coffee beans was determined on rice and ground green coffee media. A significant degree of degradation of caffeine in the green coffee medium was demonstrated with cultures of nine A. ochraceus isolates from green coffee beans. Most of these isolates showed the potential to grow on moist green coffee beans and to produce a significant amount of ochratoxins.
分别从生咖啡豆和糯米中分离得到的棕曲霉S-235-100菌株和IFM 0458菌株，在含有0.1%至1.0%咖啡因的酵母提取物-蔗糖（YES）培养基中进行生长及赭曲霉毒素A产生情况的检测。当咖啡因浓度超过0.1%时，IFM 0458菌株的菌丝体生长及赭曲霉毒素A的形成受到抑制，在咖啡因浓度为0.5%和1.0%时不产生赭曲霉毒素A。与此相反，当在YES培养基上生长时，棕曲霉S-235-100菌株在含有0.5%和1.0%咖啡因的情况下产生大量的赭曲霉毒素A，培养15至20天后达到最大值。对从生咖啡豆中分离得到的另外9株棕曲霉、3株雅致曲霉和1株核盘曲霉在大米和磨碎的生咖啡豆培养基上赭曲霉毒素A的形成情况进行了测定。从生咖啡豆中分离得到的9株棕曲霉培养物显示，生咖啡豆培养基中的咖啡因有显著程度的降解。这些分离株大多显示出在潮湿的生咖啡豆上生长并产生大量赭曲霉毒素的潜力。
