DiPaolo J A, Donovan P J
Int J Radiat Biol Relat Stud Phys Chem Med. 1976 Jul;30(1):41-53. doi: 10.1080/09553007614550791.
U.V.-exposure of foetal hamster cells (secondary or tertiary sub-cultures) seeded for colony formation resulted in the induction of transformation. The U.V.-effect on colony-forming ability resulted in a D37 of 58 ergs/mm2. The observed transformation frequency induced by U.V.-increases approximately in proportion to increased doses in the range of 7-5-60-8 ergs/mm2 examined. When cells seeded for colony formation were treated with U.V. and later with either benzo(a)pyrene or N-acetoxy-fluorenyl-acetamide, commencing 6 min after exposure to U.V., there was neither an additive nor a synergistic enhancement of the transformation frequency ordinarily observed with either chemical agent alone. In mass cultures that were X-irradiated with 250 R, seeded for colony formation and treated with either 15 or 30 ergs/mm2 of U.V. 48 hours later, the enhancement of transformation was approximately 12- and 6-fold on a colony basis and 6- and 3-fold on a dish basis, respectively. No transformation occurred after X-irradiation only. The enhancement obtained by the pre-treatment with X-ray is imilar to that reported for the combination of X-ray and chemical carcinogens.
接种用于集落形成的仓鼠胎儿细胞（二代或三代传代培养物）经紫外线照射后会诱导转化。紫外线对集落形成能力的影响导致D37为58尔格/平方毫米。在所检测的7 - 5 - 60 - 8尔格/平方毫米剂量范围内，观察到紫外线诱导的转化频率大致与剂量增加成比例增加。当接种用于集落形成的细胞先用紫外线处理，然后在紫外线照射6分钟后用苯并(a)芘或N - 乙酰氧基 - 芴基乙酰胺处理时，转化频率既没有出现单独使用任何一种化学试剂时通常观察到的相加增强，也没有协同增强。在接受250伦琴X射线照射、接种用于集落形成并在48小时后用15或30尔格/平方毫米紫外线处理的大量培养物中，基于集落的转化增强分别约为12倍和6倍，基于培养皿的转化增强分别约为6倍和3倍。仅X射线照射后未发生转化。X射线预处理所获得的增强与X射线和化学致癌物联合作用所报道的增强相似。
