Leal-Klevezas D S, Martínez-Vázquez I O, García-Cantú J, López-Merino A, Martínez-Soriano J P
Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Sierra Mojada 800, Col. Independencia, 44340, Jal. , Guadalajara, Mexico.
Vet Microbiol. 2000 Jul 3;75(1):91-7. doi: 10.1016/s0378-1135(00)00200-5.
The polymerase chain reaction (PCR) was used to diagnose goat brucellosis and compare its sensitivity against some of the most commonly used serological and bacteriological techniques. Twenty two female and one male out of 300 clinically healthy, mixed-breed goats were randomly chosen from a ranch located at Marín, Nuevo León, Mexico. Milk and blood samples were taken from each animal and used to obtain both microbiological cultures and DNA of the pathogen, and sera was tested against Rose Bengal antigen (RBT). Results showed that 86% of the blood samples were positive on the PCR test, while 60% were positive on the serological test. The pathogen was isolated from only one blood culture. Sixty four percent of the milk samples were positive on PCR tests, but failed to yield bacteria in culture. Biochemical and PCR specific assay demonstrated that Brucella abortus biovar 1 was associated with the infection. This study demonstrates the higher sensitivity of PCR over RBT and blood culture and its potential towards a rapid identification of Brucella strains.
聚合酶链反应（PCR）被用于诊断山羊布鲁氏菌病，并将其灵敏度与一些最常用的血清学和细菌学技术进行比较。从墨西哥新莱昂州马林市的一个牧场中，从300只临床健康的混种山羊中随机挑选出22只雌性和1只雄性山羊。从每只动物身上采集牛奶和血液样本，用于获得微生物培养物和病原体的DNA，并针对玫瑰红抗原（RBT）检测血清。结果显示，86%的血液样本在PCR检测中呈阳性，而60%在血清学检测中呈阳性。仅从一份血液培养物中分离出病原体。64%的牛奶样本在PCR检测中呈阳性，但在培养中未能培养出细菌。生化和PCR特异性检测表明，流产布鲁氏菌生物变种1与感染有关。这项研究证明了PCR相对于RBT和血液培养具有更高的灵敏度，以及其在快速鉴定布鲁氏菌菌株方面的潜力。
